In vitro micro-dynamic culture device of mammalian embryos and method for cultivating embryos by using same

A dynamic culture technology for mammals, applied in tissue cell/virus culture devices, biochemical equipment and methods, embryonic cells, etc. The problem of easy separation and falling off of the slices can be achieved, and the depth of the central chamber can be increased, the quality of the embryo can be improved, and the effect of avoiding adverse effects can be achieved.

Inactive Publication Date: 2014-03-19
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, in the process of use, it is found that there are still many shortcomings in this patented technology: ①The magnetic sheet and the diaphragm are easy to separate and fall off, causing the incubator to fail to work normally; ②The electromagnetic radiation generated by the coil may cause abnormal or deformed embryonic development in the later stage. To a certain extent, it has adverse effects on the development of

Method used

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  • In vitro micro-dynamic culture device of mammalian embryos and method for cultivating embryos by using same
  • In vitro micro-dynamic culture device of mammalian embryos and method for cultivating embryos by using same
  • In vitro micro-dynamic culture device of mammalian embryos and method for cultivating embryos by using same

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specific Embodiment approach 1

[0025] Specific implementation mode one: combine Figure 1~5 Describe this embodiment, the in vitro microdynamic culture device of mammalian embryo in this embodiment consists of a glass microfluidic channel layer 1, a main body layer 2, a pressure chamber sealing cover 4, an airway tube 5, a syringe 6, an electric motor 7 and a square wave generator 8 composition;

[0026] Wherein, the glass microfluidic channel layer 1 is provided with a depressed central chamber 1-1 and a depressed flow path channel 1-2, the flow path channel 1-2 communicates with the central chamber 1-1, and the depth of the depression of the central chamber 1-1 is Greater than flow channel 1-2;

[0027] The main body layer 2 is provided with an air pressure chamber 2-1, a cultivation chamber 2-2 and a diaphragm 2-3, the main body layer 2 is connected with the glass microfluidic channel layer 1, and the air pressure chamber 2-1 and the cultivation chamber 2-2 run through the main body layer 2. The cultur...

specific Embodiment approach 2

[0033] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the cross-section of the air pressure chamber 2-1 is equal in area and shape to the cross-section of the central chamber 1-1. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0034] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the cross-section of the central chamber 1-1 is circular, and the cross-sections of the air pressure chamber 2-1 and the central chamber 1-1 are coaxial circles. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses an in vitro micro-dynamic culture device of mammalian embryos and a method for cultivating embryos by using the same, relates to an in vitro culture device of embryos and a cultivation method using the same, and overcomes the defects of an in vitro oscillating micro-dynamic incubator of mammalian embryos and the defects of an existing patent technology. The culture device comprises a glass micro-fluid channel layer, a main body layer, an air chamber seal cover, an air duct, an injector, an electric motor and a square wave generator. The cultivation method comprises the following steps: 1, setting the frequency of the square wave generator and the stretching speed of the electric motor; 2, injecting a culture solution and mineral oil, and putting the embryos into a culture room; and 3, powering on a switch, and cultivating. The in vitro micro-dynamic culture device is applied to the field of biology.

Description

technical field [0001] The invention relates to an in vitro culture device for embryos and a culture method using the same. Background technique [0002] In order to simulate the internal environment of the reproductive system of female mammals, the current in vitro culture devices basically adopt swinging micro-dynamic culture. The oscillating micro-dynamic incubator is easy to cause the culture solution to spill, and such swaying and shaking will cause the embryos to continuously hit the petri dish wall of the hard petri dish, affecting the development of the embryo; moreover, the petri dish should be designed separately, and the bottom should be provided with square protrusions, In order to ensure that the culture dish will not fall or move during the cultivation process. [0003] In order to overcome the above-mentioned defects, the inventor invented the patent "a mammalian embryo in vitro culture device and its culture method" (patent number: ZL201210416329.7, applicat...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/073
CPCC12M21/06C12M23/16C12M29/14
Inventor 李井春李雁冰张莹朱思兵韩静高校委任舟启刘胜军曹阳郭丽李庆达贺显晶孙蕊
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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