Method for producing Qi yam test tube micro tuber

Abstract

The invention discloses a method for producing a Qi yam test tube micro tuber. The method comprises the steps as follows: a, preparing a Qi yam test tube seedling: cutting a tender stem at the top of a Qi yam into zonite stems, cutting the stems into small segments after the stems are disinfected on an ultra-clean workbench, putting the cut materials into a culture medium, and culturing the cut materials under an illumination condition to obtain an initial germfree test tube seedling; b, expanding and propagating the Qi yam test tube seedling: cutting the germfree test tube seedling into small segments, putting the small segments into the culture medium, and culturing the small segments under the illumination condition to obtain a test tube seedling; c, culturing the Qi yam test tube micro tuber: putting the test tube seedling into the culture medium, and culturing the test tube seedling under the illumination condition to obtain the Qi yam test tube micro tuber; d, harvesting the Qi yam test tube micro tuber. The micro tuber produced by the method disclosed by the invention is high in forming rate and is up to 97.50 percent; the method is feasible and convenient to operate; the production period is shortened, the production efficiency is improved, and the production cost is reduced; a large number of the Qi yam test tube micro tubers can be quickly cultivated; a demand on large-scale planting is met.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for efficiently producing test-tube microtubers of Qiyam yam, which is suitable for rapid breeding of detoxified Qiyam yam seeds. Background technique [0002] Yam belongs to the genus Dioscorea (Dioscoreaceae) of the order Liliales (Liliales) Dioscorea L.) Dioscorea ( Dioscorea opposita Thunb.) and its close relatives are the general term for underground tubers, which are 1-year or perennial, monocotyledonous winding vines with characteristic characteristics of dicotyledonous plants. Yam is rich in starch, polysaccharides, mucus protein, minerals and other nutrients. It is an economic crop with both food and medicine. Countries such as China, the United States, the United Kingdom, and Japan all list yam in the Pharmacopoeia. Yam has a mild smell, warm but not sudden, slightly fragrant but not dry. It is a good medicine for invigorating t...

AI Technical Summary

Problems solved by technology

But there is no report about the method of 'Qi Yam' efficiently forming test tube micro tubers by solid medium, the purpose of this invention is to establish the method of 'Qi Yam' efficiently forming test tube micro tubers in vitro, and solve the problem by rapid propagation of test tube micro tubers In the production of "Qishan medicine" detoxification seed medicine, the production cycle is long, the supply exceeds the demand, and the genetic stability of the variety is maintained

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