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Method for extracting squalene by treating eucalyptus urophylla with complex enzyme method

A compound enzyme method and squalene technology, which is applied in chemical instruments and methods, hydrocarbons, hydrocarbon purification/separation, etc., can solve the problems of complex process steps, large amount of solvent, and unfavorable environment and human health. Achieve the effects of improving economic value, simplifying extraction and separation steps, and reducing usage

Inactive Publication Date: 2014-03-26
WUHAN SHUTAI TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process steps are complicated and the amount of solvent used is relatively large, which is not conducive to the environment and human health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for extracting squalene from Eucalyptus macrocarpa by a compound enzyme method: cut 50 kg of Eucalyptus macrocephalus into small sections of 1-2 cm, add 50 kg of water, add 100 g of compound enzymes to the solution, and the ratio of the compound enzymes is alkaline Protease (2709): cellulase: ligninase: pectinase = 7:1.5:1:0.5, heat up at 50-70°C, keep warm and stir for 3-9 hours, add 50kg of 95% ethanol, add 150kg of n-hexane , Carry out extraction to obtain squalene crude extract 1500g, after molecular distillation, obtain the squalene 280g that content is 85%.

Embodiment 2

[0019] A method for extracting squalene from Eucalyptus grandis by compound enzyme method: cut 50 kg of Eucalyptus grandis into 1-2 cm segments, add 50 kg of water, add 75 g of compound enzymes to the solution, and the ratio of the compound enzymes is alkaline Protease (2709): cellulase: ligninase: pectinase = 7:1.5:1:0.5, heat up at 50-70°C, keep warm and stir for 3-9 hours, add 25kg of 95% ethanol, add 150kg of n-hexane , extract and obtain squalene crude extract 1480g, after molecular distillation, obtain the squalene 295g that content is 87%.

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PUM

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Abstract

The invention discloses a method of treating eucalyptus urophylla to extract squalene by a complex enzyme method. The method comprises the following steps: (1), preparing materials; (2), carrying out complex enzyme treatment; (3), extracting; (4), carrying out molecular distillation; and (5), collecting squalene. The method disclosed by the invention has the advantages that usage amount of a solvent can be reduced, extracting and separating steps are simpler and industrial production needs are satisfied; and meanwhile, an industry chain of the wood industry and papermaking-pulping can be prolonged, and economic value of the eucalyptus urophylla is improved.

Description

technical field [0001] The invention relates to a method for extracting squalene by treating Eucalyptus grandis with a compound enzyme method. Specifically, it involves the application of compound enzymes to extract squalene in Eucalyptus grandis wood. Background technique [0002] Squalene is a lipid unsaponifiable substance, originally discovered from shark liver oil, named Squalene in 1914, and its chemical name is 2,6,10,15,19,23-hexamethyl-2 ,6,10,14,18,22-Tetracosahexaene, an open-chain triterpene, also known as cod liver oil terpene, can improve the activity of superoxide dismutase (SOD) in the body, enhance the body's immunity, and improve sexual function. , Anti-aging, anti-fatigue, anti-tumor and other physiological functions, it has liver protection effect, promotes liver cell regeneration and protects liver cells, thereby improving liver function. Anti-fatigue and enhance the body's disease resistance, improve human immune function. Protect the function of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C11/21C07C7/00
Inventor 简晓红
Owner WUHAN SHUTAI TECH
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