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Method for preparing endogenous retrovirus polyclonal antibody

A retrovirus, polyclonal antibody technology, applied in the field of experiments, can solve problems such as high infection risk, and achieve the effect of low cost and simple experiment

Inactive Publication Date: 2014-03-26
仲集华
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Problems solved by technology

|Three I itHhf? The porcine fibroblasts that inhibit the expression of PERV are all derived from German white pigs with high expression of PERV mRNA. After silencing by RNA interference technology,111 its expression level decreased, but there was an "off-target" in the RNA interference technology effect", so this kind of nuclear donor cells with high expression background of PERV mRNA still has a higher potential risk of infection to a certain extent

Method used

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Embodiment Construction

[0008] A method for preparing endogenous retrovirus polyclonal antibodies, the steps are as follows: inducing gene fragments into fatty acid binding proteins; then recombining proteins by particle exchange; high-purity purification; obtaining the required high-purity albumin; and then Improving the immune test; adding decolorization solution; 90ml of ultrapure water can be added to the decolorization solution, and the recombinant protein can specifically react with the monoclonal antibody protein.

[0009] In the present invention, the protein needs to be purified and separated from antiserum components.

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Abstract

The invention discloses a method for preparing an endogenous retrovirus polyclonal antibody. The method comprises the following steps: inducing a gene segment into a fatty acid binding protein; recombining the protein with a particle exchange process; carrying out high-purity purification to obtain the required high-purity albumin; carrying out improved immunity test; and adding a destaining solution, wherein 90ml of ultrapure water can be added into the destaining solution, the recombinant protein can generate specific reaction with the monoclonal antibody protein, and the protein needs to be purified and separated from antiserum. The method has the advantages of simple experiment and lower cost; and the preparation of the antibody has important meanings in detection of expression and transmission of PERV (porcine endogenous retrovirus) in the clinical organs and artificial liver in future.

Description

technical field [0001] The invention relates to an experimental method, specifically, a method for preparing endogenous retrovirus polyclonal antibody. Background technique [0002] Species transplantation provides an extremely rich source of organs, tissues and cells in the treatment of human diseases. Because the source of pigs is convenient, easy to breed, and has a high similarity to human anatomy and life, it can select organs of appropriate size and rich and functional liver cells, so it has become the first choice for breeding transplants and bioartificial livers. However, for xenotransplantation and bioartificial liver, porcine endogenous retrovirus (porcine endogenous retrovirus, PERV) exists in pig organs, tissues and cells as xenogeneic sources, PERV is a Y retrovirus, It is integrated in the pig chromosome; the PERV copy number and mRNA expression vary greatly depending on the species. |Three I itHhf? The porcine fibroblasts that inhibit PERV expression are al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10
Inventor 仲集华
Owner 仲集华
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