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A kind of colorectal cancer screening kit

A kit and technology for colorectal cancer, applied in biological testing, biochemical equipment and methods, material inspection products, etc., can solve the problems of low sensitivity and specificity, high cost, and large number of detection genes

Active Publication Date: 2016-05-25
BIOMERIEUX SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the above detection methods, when the number of detected genes is small, the sensitivity and specificity are low, while when the sensitivity and specificity are high, the number of detected genes is too large and the cost is high

Method used

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  • A kind of colorectal cancer screening kit
  • A kind of colorectal cancer screening kit
  • A kind of colorectal cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Example 1 sample collection

[0117] After informed consent, peripheral blood samples were collected from 187 colorectal cancer patients (CRC) confirmed by clinicopathological diagnosis and 173 colonoscopy-negative control patients (CNC), collected between 2006 and 2010.

[0118] CRC was recruited from the Department of Colorectal Surgery, Shanghai Cancer Center, Fudan University, China, and all patients were staged according to the TNM staging system recommended by the International Union Against Cancer (UICC). None of the patients received preoperative chemotherapy or radiotherapy. Patients with hereditary colorectal cancer or inflammatory bowel disease (Corhn's disease or ulcerative colitis) were excluded from the present invention. CNCs were recruited from Shanghai Community Hospital and passed colonoscopy without any symptoms of colorectal cancer or polyps. The demographic and clinical characteristics of the test samples are shown in Table 1:

[0119] Table 1 Th...

Embodiment 2D

[0124] Example 2 Detection of expression levels of 18 genes such as DUSP2

[0125] 1. Experimental method

[0126] (1) Selection of housekeeping genes

[0127] The geometric mean of the expression levels of CSNK1G2, DECR1, and FARP1 was used as the "meta-housekeeping gene" as a correction factor for real-time quantitative PCR data.

[0128] (2) RNA extraction and real-time quantitative PCR detection

[0129] Whole blood draw: Take 2.5ml of peripheral blood from each participant in PAXgene TM Blood RNA tubes (PreAnalytix GmbH, Hombrechtikon, CH) were processed according to the instructions;

[0130] Total RNA extraction: according to the instructions, use PAXgene TM Blood RNA system (PreAnalytix) was used to extract total RNA; the amount of total RNA was detected with a spectrophotometer at an OD value of 260nm, and the RNA6000Nano on an Agilent bioanalyzer A kit (Agilent Technologies, Palo Alto, CA, U.S.A.) was used to detect the quality of total RNA, and RNA with an int...

Embodiment 3

[0199] Example 3 Correlation between gene expression and colorectal cancer

[0200] 1. Correlation between single gene expression and colorectal cancer

[0201] According to the method of Example 2, the expression levels of 18 genes in the blood samples of the 200 participants in Group 1 (including 100 CRCs and 100 CNCs) in Example 1 were detected respectively - relative expression levels, T test and calculation of the control group The ROC curve was established based on the average relative expression level of the tumor group, and the area under the curve (AUC) of the ROC curve was calculated. AUC can reflect the correlation between the gene and colorectal cancer.

[0202] (1) DUSP2 (dual specificity phosphate 2):

[0203]

[0204] The correlation between the expression level of DUSP2 gene and colorectal cancer is 0.75, and the average expression level difference between the control group and the tumor group is significant, P<0.01, low expression of this gene can signific...

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Abstract

The invention discloses a colorectal cancer screening kit, which comprises related reagents for detecting expression level of any one or more of 18 genes provided by the invention. The invention also discloses an application of 18 genes in preparation of a colorectal cancer screening reagent. The invention further discloses a detection method of 18 genes, a detection kit and a colorectal cancer screening method. The kit disclosed by the invention can accurately detect whether a sample to be detected gets colorectal cancer, and has a good prospect in clinical application.

Description

technical field [0001] The invention relates to a colorectal cancer screening kit. Background technique [0002] Colorectal cancer is one of the most common malignant tumors. It ranks fifth in the incidence of cancer in the United States, ranks fourth in the incidence of cancer in China, and is the third leading cause of cancer death in Europeans. When colorectal cancer is at an early stage, its cure rate is high. Early screening of colorectal cancer is the key to successful treatment and patient survival, and it is also a major challenge in the field of public health. [0003] Traditional testing methods include fecal occult blood test, sigmoidoscopy, colonoscopy, double-contrast barium meal, and digital rectal examination. The patient compliance of these examinations is low and the operation is complicated. It is necessary to find a method with high patient compliance and easy operation. [0004] Looking for biomarkers in peripheral blood to detect colorectal cancer is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N33/68G01N33/53C12N15/11
CPCC12Q1/6886C12Q2600/158G01N33/57446G01N33/57484G01N2800/50
Inventor 叶迅孟夏徐清华刘芳吴非
Owner BIOMERIEUX SA
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