Refining method of l-glutamine

A technology for glutamine and microbial fermentation, which is applied in the field of extraction and refining of amino acid derivative fermentation liquid, can solve the problems of pollution, low production capacity and output value, etc., and achieves the effects of reducing production cost, dosage and energy consumption.

Inactive Publication Date: 2016-08-24
SHANDONG MINQIANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because L-glutamine not only has important scientific research, practical production and application value, but also plays an important role in physiological functions of L-glutamine, and the current domestic traditional production methods are still seriously polluted and the production capacity is low. Therefore, Approved by the State Council and representatives of the National People's Congress, the production of L-glutamine by fermentation has been included in the national "Eleventh Five-Year Plan" key scientific and technological projects

Method used

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  • Refining method of l-glutamine
  • Refining method of l-glutamine
  • Refining method of l-glutamine

Examples

Experimental program
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Effect test

preparation example Construction

[0059] (1) Preparation of fermentation broth:

[0060] Brevibacterium flavum MQG121 strain is used as the fermentation strain;

[0061] (1) Inclined surface culture: insert the bacteria in the amber tube into the sterile slanted medium at 28-32°C for 20-24 hours;

[0062] (2) Shake flask strains: insert the cultured slant strains into sterile shake flask seed medium at 28-32°C for 16-20 hours;

[0063] (3) Primary seed culture: Put the cultured shake flask seed liquid into the sterile primary seed medium, under the condition of 1:0.5 ventilation, stirring at 240rpm, and cultivate at 28-32°C for 12-16 hours;

[0064] (4) Secondary seed culture: put the cultivated primary seed solution into the sterile secondary seed medium, under 1:0.5 ventilation, stirring at 220rpm, and cultivate at 28-32°C for 2-6 hours;

[0065] (5) Inoculate the cultured secondary seeds into the sterilized fermentation medium, under the ventilation condition of 1:0.3, the stirring speed is 140rpm, adjust...

Embodiment 2

[0093] The extraction and refining method of L-glutamine, the method comprises the following steps:

[0094] (1) Preparation of fermentation broth:

[0095] Brevibacterium flavum MQG121 strain is used as the fermentation strain;

[0096] Slant culture: put the bacteria in the amp tube into the slant medium and culture at 30°C for 22 hours;

[0097] Shake flask strain: insert the cultured slant strain into the shake flask seed medium for 18 hours at 30°C;

[0098] First-level seed culture: put the cultivated shake flask seed solution into the sterilized first-level seed medium and cultivate at 30°C for 14 hours;

[0099] Secondary seed culture: put the cultivated primary seed solution into the sterilized secondary seed medium and cultivate it at 30°C for 4 hours; inoculate the cultivated secondary seed into the sterilized fermentation wind, adjust the pH value to 6.6, and obtain a fermentation liquid containing glutamine products through 45 hours of cultivation;

[0100] Ab...

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Abstract

The invention belongs to the technical field of extraction and refinement of amino acid derivative fermentation liquids, and in particular relates to a method for extracting and refining L-glutamine. The method comprises the following steps: preparing a fermentation liquid, namely performing slant culture, performing shake-flask culture, performing primary seed culture, performing secondary seed culture, and continuing to ferment; extracting and refining a fermented product, namely preprocessing the fermentation liquid, performing ion exchange, performing secondary crystallization, performing color and impurity removal, performing color and impurity removal and concentration by a nanofiltration membrane, performing concentration and crystallization, and drying to obtain a finished product. The method has the benefits that organic and inorganic matters and pigments in the subsequent extracting and refining liquid of the glutamine fermentation liquid are remarkably reduced and the glutamine yield is increased; the product quality is improved, the production cost is reduced, and the labor intensity of workers is reduced to the great extent; the discharge amount of solid and liquid wastes such as water, acid, alkali and active carbon is greatly reduced and the environmental pollution is alleviated.

Description

technical field [0001] The invention belongs to the technical field of extraction and refining of amino acid derivative fermentation liquid, and in particular relates to a method for extracting and refining L-glutamine. Background technique [0002] Glutamine (Glutamin), also known as glutamic acid, is the most abundant non-essential amino acid in the human body, and it is the only amino acid that can directly pass through the cerebrovascular barrier (BBB). In the human body, it is stored in skeletal muscle or blood. When injured or ill, glutamine may need to be obtained in sufficient quantities by consuming Gln-containing foods. Organisms catalyze the reaction of glutamic acid and ammonium salts to produce glutamine through glutamine synthetase. [0003] L-Glutamine, this product is used as nutritional supplement and flavoring supplement in food processing. And L-Glutamine is an important nutritional supplement for bodybuilders and bodybuilders. It (hereinafter referred...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/14C07C237/06C07C231/24C12R1/13
Inventor 高树营胡安红
Owner SHANDONG MINQIANG BIOTECH
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