Application of PIK3C2G in evaluation and detection kit for curative effect of colorectal cancer chemotherapy

A technology for colorectal cancer and kits, applied in the fields of molecular biology and tumor biology, able to solve problems such as possible and specific applications that have not been studied

Active Publication Date: 2014-04-09
浙江自贸区锐赛生物医药科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Interestingly, the expression of PIK3C2G in leukemia cell homing was inhibited by Bcr-Abl, suggesting that its expression inhibition is related to cell migration (Yu W, Sun X, Tang H, Tao Y, Dai Z (2010) Inhibition of class II phosphoinositide3- kinase gamma expression by p185(Bcr-Abl)contributes to impaired chemotaxis and aberrant homing of leukemic cells.Leuk Lymphoma51:1098-1107), suggesting that PIK3C2G has the possibility of becoming a potential tumor marker, but whether PIK3C2G in colorectal cancer Potential and specific applications with biomarkers not investigated

Method used

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  • Application of PIK3C2G in evaluation and detection kit for curative effect of colorectal cancer chemotherapy
  • Application of PIK3C2G in evaluation and detection kit for curative effect of colorectal cancer chemotherapy
  • Application of PIK3C2G in evaluation and detection kit for curative effect of colorectal cancer chemotherapy

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specific Embodiment

[0022] Collection of samples and their follow-up information

[0023] The inventor has collected colorectal cancer patient samples from Ruijin Hospital since 2006, and selected 92 samples (35 cases with a survival period of less than five years, and 57 cases with a survival period of more than five years) of stage III colorectal cancer tumors after data collation and follow-up. Paraffin embedded tissue samples.

[0024] Paraffin sample DNA extraction

[0025] Cut 5 mg of sample from paraffin-embedded tissue, put it into a 2 ml centrifuge tube, add 1 ml of xylene, and vortex for 10 seconds. Centrifuge at room temperature for 2 minutes at 14,000 rpm. Discard the supernatant. Add 1 ml of absolute ethanol to wash the precipitate, vortex, centrifuge at 14,000 rpm for 2 minutes at room temperature, and discard the supernatant. Place the centrifuge tube in an ultra-clean bench to remove excess ethanol. Add 180 μl RTL buffer (Qiagen), 20 μl proteinase K, mix well, incubate at 56°...

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Abstract

The invention relates to the fields of molecular biology and phymatology, and discloses an application of a biomarker associated with a colorectal cancer. The marker is PIK3C2G, and particularly, deoxyribonucleic acid (DNA) of the marker is inversely related to survival time after colorectal cancer occurrence and chemotherapy of a sufferer relative to copy number variation. The invention also relates to a primer pair, a kit and a method for detecting the PIK3C2G in a colorectal cancer, the primer pair for detecting the PIK3C2G and a primer pair for detecting a reference gene reduced glyceraldehyde-phosphate dehydrogenase(GAPDH) are contained in the kit of the PIK3C2G. An upstream primer base sequence is shown in SEQ ID NO:1. The primer can be applied to detection of the copy number variation of the PIK3C2G through polymerase chain reaction (PCR) amplification, so that detection of the colorectal cancer and evaluation of the curative effect of oxaliplatin (FOLFOX) chemotherapy of the colorectal cancer through the PIK3C2G become possible.

Description

[technical field] [0001] The invention belongs to the fields of molecular biology and tumor biology, relates to a colorectal cancer gene marker and its application, and at the same time, relates to a primer pair, a kit and a method for detecting PIK3C2G in colorectal cancer. [Background technique] [0002] Colorectal cancer is one of the most common malignant tumors, and its incidence rate in China is on the rise, ranking third among all malignant tumors, posing a serious threat to human health. [0003] With the development of molecular analysis methods, various new diagnosis and treatment methods and new drugs are emerging, and the treatment effect of colorectal cancer has been significantly improved. However, although traditional clinicopathological factors can provide certain efficacy information for colorectal cancer patients, the extent to which these risk factors influence colorectal cancer prognosis cannot be well quantified and integrated. For individual patients, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/106
Inventor 李华光汤二将陆星亦张腾林谋斌
Owner 浙江自贸区锐赛生物医药科技有限公司
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