Preparation method of peptide ligand for non-competitive detection of water-soluble pesticide residues

Abstract

The invention discloses a preparation method of a peptide ligand for non-competitive detection of water-soluble pesticide residues. The preparation method comprises the following steps: coupling beta-cyclodextrin (beta-CD) with bovine serum albumin (BSA), enveloping the coupling products with a solid phase carrier to complex with chlorpyrifos; performing four-round screening on commercialized phage display peptide libraries by using 'positive and negative alternative screening' strategy; separating and selecting mono phage clones from the fourth-round screening elution products, identifying the mono phage clone capable of specifically recognizing 'beta-CD-pesticide' complex; finally sequencing the identified phage clone to determine the insertion of polypeptide fragment and obtain the amino acid sequence information of the polypeptide. The preparation method has the advantages that the molecular modification of the detection object is not needed, and the obtained peptide ligand is used for constructing the non-competitive detection of water-soluble pesticide residues.

Description

technical field [0001] The invention relates to a method for preparing a peptide ligand for non-competitive detection of fat-soluble pesticide residues, belonging to the field of biotechnology and analysis and detection technology. Background technique [0002] The problem of pesticide residues is one of the food safety and environmental safety issues that are widely concerned. The premise of its control is to establish an effective detection technology system. The use of rapid detection methods to conduct preliminary screening of possible pesticide residues in food or the environment is recognized by domestic and foreign counterparts as an important part of the pesticide residue detection system. Although many studies have been carried out on the immunological rapid detection of pesticide residues, there are not many examples of its practical application so far. The cumbersome antibody preparation process and the single analysis and detection mode are the main bottleneck f...

AI Technical Summary

Problems solved by technology

In the process of preparing antibodies using phage display technology, it is usually necessary to construct a high-capacity phage display antibody library first. However, the process of antibody library construction is usually cumbersome, and the high-capacity requirement is usually difficult to meet

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