Preparation method of peptide ligand for non-competitive detection of water-soluble pesticide residues

A fat-soluble pesticide, non-competitive technology, applied in the direction of peptides, etc., can solve the problems of difficult to achieve high-capacity requirements, cumbersome process of antibody library construction, etc., to achieve the effect of shortening the preparation cycle, simple and effective operation, and improving screening efficiency.
CN103724401BActive Publication Date: 2015-06-10HUNAN UNIV OF ARTS & SCI +1
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUNAN UNIV OF ARTS & SCI
Publication Date
2015-06-10

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a preparation method of a peptide ligand for non-competitive detection of water-soluble pesticide residues. The preparation method comprises the following steps: coupling beta-cyclodextrin (beta-CD) with bovine serum albumin (BSA), enveloping the coupling products with a solid phase carrier to complex with chlorpyrifos; performing four-round screening on commercialized phage display peptide libraries by using 'positive and negative alternative screening' strategy; separating and selecting mono phage clones from the fourth-round screening elution products, identifying the mono phage clone capable of specifically recognizing 'beta-CD-pesticide' complex; finally sequencing the identified phage clone to determine the insertion of polypeptide fragment and obtain the amino acid sequence information of the polypeptide. The preparation method has the advantages that the molecular modification of the detection object is not needed, and the obtained peptide ligand is used for constructing the non-competitive detection of water-soluble pesticide residues.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for preparing a peptide ligand for non-competitive detection of fat-soluble pesticide residues, belonging to the field of biotechnology and analysis and detection technology. Background technique

[0002] The problem of pesticide residues is one of the food safety and environmental safety issues that are widely concerned. The premise of its control is to establish an effective detection technology system. The use of rapid detection methods to conduct preliminary screening of possible pesticide residues in food or the environment is recognized by domestic and foreign counterparts as an important part of the pesticide residue detection system. Although many studies have been carried out on the immunological rapid detection of pesticide residues, there are not many examples of its practical application so far. The cumbersome antibody preparation process and the single analysis and detection mode are the main bottleneck f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More