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Method for quantitatively detecting polysaccharide of semen cassiae

A quantitative detection method, cassia seed technology, applied in the field of quantitative detection of cassia seed polysaccharides, can solve problems such as large deviation of true value

Active Publication Date: 2014-04-16
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Secondly, cassia seed polysaccharides are composed of various monosaccharides according to a certain ratio, while the existing methods generally use glucose as a standard substance to obtain the content of cassia seed polysaccharides by establishing a standard curve, so the results often deviate greatly from the true value

Method used

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  • Method for quantitatively detecting polysaccharide of semen cassiae
  • Method for quantitatively detecting polysaccharide of semen cassiae
  • Method for quantitatively detecting polysaccharide of semen cassiae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Get 5 grams of cassia seeds and pulverize through a 20-mesh sieve to obtain a sample cassia seed powder.

[0034] Soak the cassia seed powder with acetone according to the ratio of solid to liquid 1:15 (w / w), shake it for 30 minutes, and let it stand for 30 minutes; pour off the supernatant liquid, and then repeat the operation according to the same ratio of solid to liquid until the upper layer is colorless and the solid and liquid are separated Obtain cassia seed degreasing powder.

[0035] The cassia seed defatted powder treated with acetone was extracted with hot water at 80°C for 3 hours according to the solid-liquid ratio of 1:35 (g / mL), and the filtrates were combined to obtain the extract.

[0036] PVPP / alcohol precipitation treatment: Add PVPP to the extract at a concentration of 0.5g / 100ml, stir slowly for 1 hour, filter, and combine the filtrates. Add absolute ethanol equivalent to three times the volume of the filtrate to the filtrate, let it stand at room ...

Embodiment 2

[0039] Get 5 grams of cassia seeds and pulverize through a 40-mesh sieve to obtain a sample cassia seed powder.

[0040] Soak the cassia seed powder with acetone according to the ratio of solid to liquid 1:15 (w / w), shake it for 30 minutes, and let it stand for 30 minutes; pour off the supernatant liquid, and then repeat the operation according to the same ratio of solid to liquid until the upper layer is colorless and the solid and liquid are separated Obtain cassia seed degreasing powder.

[0041] The cassia seed defatted powder treated with acetone was extracted with hot water at 80°C for 3 hours according to the solid-liquid ratio of 1:35 (g / mL), and the filtrates were combined to obtain the extract.

[0042]PVPP / alcohol precipitation treatment: Add PVPP to the extract at a concentration of 2g / 100ml, stir slowly for 2 hours, filter, and combine the filtrates. Add anhydrous ethanol equivalent to three times the volume of the filtrate to the filtrate, let it stand at room t...

Embodiment 3

[0045] Get 5 grams of cassia seeds and pulverize through a 40-mesh sieve to obtain a sample cassia seed powder.

[0046] Soak the cassia seed powder with acetone according to the ratio of solid to liquid 1:15 (w / w), shake it for 30 minutes, and let it stand for 30 minutes. Pour off the supernatant liquid, and then repeat the operation according to the same ratio of material to liquid until the upper layer is colorless, and the solid and liquid are separated to obtain the degreased cassia seed powder.

[0047] The cassia seed defatted powder treated with acetone was extracted with hot water at 80°C for 3 hours according to the solid-liquid ratio of 1:35 (g / mL), and the filtrates were combined to obtain the extract.

[0048] PVPP / alcohol precipitation treatment: Add PVPP to the extract at a concentration of 2g / 100ml, stir slowly for 2 hours, filter, and combine the filtrates. Add anhydrous ethanol equivalent to three times the volume of the filtrate to the filtrate, let it stan...

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Abstract

The invention belongs to the field of food and pharmaceutical analysis and discloses a method for quantitatively detecting polysaccharide of semen cassiae. The semen cassiae used as a raw material is crushed and soaked by acetone to realize degreasing and decoloring, then is subjected to hot water extraction, filtering, concentration, alcohol precipitation and polyvinylpyrrolidone treatment to remove polyphenols and other proteins, and finally, is centrifuged and realizes constant volume to obtain the polysaccharide of the semen cassiae. A standard mixed monosaccharide sample is prepared according to the molar ratio of monosaccharides forming the polysaccharide, a standard curve regression equation is obtained, and the content of the polysaccharide of the semen cassiae is determined by a developing process. The method can quantitatively detect the polysaccharide of the semen cassiae accurately and rapidly.

Description

technical field [0001] The invention belongs to the field of food and drug analysis, and in particular relates to a quantitative detection method of cassia seed polysaccharide. Background technique [0002] At present, the methods used to determine the polysaccharide content of Semen Cassiae mainly use polysaccharide methods to determine the polysaccharide content of Semen Cassiae: including chromogenic method, isotope labeling method, immunological method, biological detection method, high performance liquid chromatography-differential detection method and evaporative light Scattering detection method, etc. The chromogenic method does not require large instruments, is simple to operate, and is easy to meet the needs of rapid determination, so it is widely used in the detection of polysaccharides from Semen Cassiae. For example, anthrone-sulfuric acid method (Zhong Xianfeng, Xie Mingyong, Huang Guidong, Extraction of Active Components from Semen Cassiae. Journal of Nanchang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 姚松君高新开熊斌
Owner INFINITUS (CHINA) CO LTD
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