Tissue culture method for German iris tectorum

A technique of tissue culture and iris, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of increasing the speed of reproduction, improving the uniformity of seedlings, and improving the traits of the female parent

Inactive Publication Date: 2014-04-23
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No report on tissue cu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for tissue culture of Iris germanica, the method comprises the following steps:

[0035] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0036] 1) Bud induction medium: MS+6-BA5mg / L+NAA5mg / L;

[0037] 2) Adventitious bud proliferation medium: MS+6-BA3mg / L+NAA0.3mg / L;

[0038] 3) Strong seedling medium: MS+6-BA0.1mg / L+NAA0.1mg / L;

[0039] 4) Rooting medium: MS+NAA0.1mg / L;

[0040] For the pH5.8 of the above-mentioned medium, add 30g / .L sucrose, 6g / L agar, culture temperature is (25±1)℃, light is about 80μmol ms:

[0041] (2) Tissue culture of Iris germanica

[0042] 1) Obtaining sterile materials

[0043] Pick german iris buds, wash them with tap water for 2 hours, put them on a clean bench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, rinse them with sterile water for 5-6 times, and blot the surface with sterile filter paper ...

Embodiment 2

[0053] A method for tissue culture of Iris germanica, the method comprises the following steps:

[0054] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0055] 1) Bud induction medium: MS+6-BA1.0mg / L+NAA0.1mg / L;

[0056] 2) Adventitious bud proliferation medium: MS+6-BA1mg / L+NAA0.1mg / L;

[0057] 3) Strong seedling medium: MS+6-BA0.5mg / L+NAA0.1mg / L;

[0058] 4) Rooting medium: MS+NAA0.05mg / L;

[0059] For the pH5.8 of the above-mentioned medium, add 30g / .L sucrose, 6g / L agar, culture temperature is (25±1)℃, light is about 70μmol ms:

[0060] (2) Tissue culture of Iris germanica

[0061] 1) Obtaining sterile materials

[0062]Pick german iris buds, wash them with tap water for 2 hours, put them on a clean bench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, rinse them with sterile water for 5-6 times, and blot the surface with sterile filter pap...

Embodiment 3

[0072] A method for tissue culture of Iris germanica, the method comprises the following steps:

[0073] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0074] 1) Bud induction medium: MS+6-BA3.0mg / L+NAA0.3mg / L;

[0075] 2) Adventitious bud proliferation medium: MS+6-BA2mg / L+NAA0.2mg / L;

[0076] 3) Strong seedling medium: MS+6-BA2mg / L+NAA0.2mg / L;

[0077] 4) Rooting medium: MS+NAA0.2mg / L;

[0078] For the pH5.8 of the above-mentioned medium, add 30g / .L sucrose, 6g / L agar, culture temperature is (25±1)℃, light is about 90μmol ms:

[0079] (2) Tissue culture of Iris germanica

[0080] 1) Obtaining sterile materials

[0081] Pick german iris buds, wash them with tap water for 2 hours, put them on a clean bench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, rinse them with sterile water for 5-6 times, and blot the surface with sterile filter paper...

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Abstract

The invention relates to a tissue culture method for German iris tectorum. The method comprises the following steps: (1) preparation of culture media: including the following ingredients and contents in each tissue culture stage: 1) bud inducing culture media: MS, and 1.0-5.0 mg/L of 6-BA and 0.1-0.5 mg/L of NAA; 2) adventitious bud proliferation culture media: MS, and 1.0-3.0 mg/L of 6-BA and 0.1-0.3 mg/L of NAA; 3) strong seedling culture media: MS, and 0.5-2.0 mg/L of 6-BA and 0.1-0.2 mg/L of NAA; 4), rooting culture media: MS and 0.05-0.2 mg/L of NAA; controlling the ph of the culture media under each condition to 5.8, adding 30 g/L of glucose and 6g/L of agar, and culturing at a temperature of 26 DEG C or 24 DEG C under the solar irradiation of about 80 micromol/ms. Compared with the prior art, the tissue culture method has the advantages that the reproduction speed and the tidiness of seedlings are greatly improved, and original female parent properties are better kept.

Description

technical field [0001] The invention relates to plant cultivation, in particular to a tissue culture method of German iris. Background technique [0002] German iris is a perennial herb of the family Iridaceae, native to central and southern Europe. It is now widely planted all over the world. Plant height 50CM. It has thick and fat rhizomes, clusters of sword-shaped leaves, slightly grayish green, purple and white flowers, and the flowering period is May-June. German irises have large, elegantly colored flowers. Hi warm, slightly humid and sunny environment. Cold-resistant, dry and semi-shady, afraid of stagnant water. It should be loose, fertile and well-drained calcareous soil. Iris plants have strong cold resistance, strong growth, beautiful leaves, large and colorful flowers, and are excellent flower viewing ground covers. This variety is widely used in gardens, suitable for potted plants, flower beds, and flower borders. It is also an important cut flower Materi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄建荣张浪陈建华沈勤
Owner 上海上房园艺有限公司
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