Double-labeled immunohistochemical staining kit for micro invasive lung adenocarcinoma

A technique for immunohistochemistry and staining reagents, which is used in measuring devices, instruments, disease diagnosis, etc., to achieve the effects of short experiment time, fewer staining steps, and intuitive comparison of experimental results.

Active Publication Date: 2014-04-23
FUZHOU MAIXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Based on our findings, we designed a CD34 and β-Tubulin-Ⅲ double-labeled immunohistochemical staining kit for displaying the invasive and non-invasive components of

Method used

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  • Double-labeled immunohistochemical staining kit for micro invasive lung adenocarcinoma
  • Double-labeled immunohistochemical staining kit for micro invasive lung adenocarcinoma
  • Double-labeled immunohistochemical staining kit for micro invasive lung adenocarcinoma

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The research objects were formaldehyde-fixed-paraffin-embedded microinvasive lung adenocarcinoma tissues (Department of Pathology, Fuzhou General Hospital of Nanjing Military Region). The steps of immunohistochemical experiment are as follows:

[0028] (1) The tissue slices were dry-baked in a 67°C incubator for 2 hours.

[0029] (2) Conventional xylene dewaxing 3 times, 6 minutes each time, hydration in 100%, 100%, 95%, 85% gradient ethanol, 3 minutes each time, and finally rinsed with tap water.

[0030] (3) High-pressure repair in citric acid antigen retrieval solution of pH 6.0 for 1.5-2 minutes, naturally cool to room temperature, rinse with tap water, and rinse with PBS for 3×3 minutes.

[0031] (4) Incubate in 3% hydrogen peroxide at room temperature for 10 minutes to block endogenous peroxidase, wash with PBS for 3×3 minutes.

[0032] (5) Block with normal animal serum for 10 minutes, shake off excess serum without washing, add CD34 and β-Tubulin-Ⅲ mixed prima...

Embodiment 2

[0039] The research objects were formaldehyde-fixed-paraffin-embedded microinvasive lung adenocarcinoma tissues (Department of Pathology, Fuzhou General Hospital of Nanjing Military Region). The steps of immunohistochemical experiment are as follows:

[0040] (1) The tissue slices were dry-baked in a 67°C incubator for 2 hours.

[0041] (2) Conventional xylene dewaxing 3 times, 6 minutes each time, hydration in 100%, 100%, 95%, 85% gradient ethanol, 3 minutes each time, and finally rinsed with tap water.

[0042] (3) High-pressure repair in citric acid antigen retrieval solution of pH 6.0 for 1.5-2 minutes, naturally cool to room temperature, rinse with tap water, and rinse with PBS for 3×3 minutes.

[0043] (4) Incubate in 3% hydrogen peroxide at room temperature for 10 minutes to block endogenous peroxidase, wash with PBS for 3×3 minutes.

[0044] (5) Block with normal animal serum for 10 minutes, shake off excess serum without washing, add CD34 and β-Tubulin-Ⅲ mixed prima...

Embodiment 3

[0051] The research objects were formaldehyde-fixed-paraffin-embedded microinvasive lung adenocarcinoma tissues (Department of Pathology, Fuzhou General Hospital of Nanjing Military Region). The steps of immunohistochemical experiment are as follows:

[0052] (1) The tissue slices were dry-baked in a 67°C incubator for 2 hours.

[0053] (2) Conventional xylene dewaxing 3 times, 6 minutes each time, hydration in 100%, 100%, 95%, 85% gradient ethanol, 3 minutes each time, and finally rinsed with tap water.

[0054] (3) High-pressure repair in citric acid antigen retrieval solution of pH 6.0 for 1.5-2 minutes, naturally cool to room temperature, rinse with tap water, and rinse with PBS for 3×3 minutes.

[0055] (4) Incubate in 3% hydrogen peroxide at room temperature for 10 minutes to block endogenous peroxidase, wash with PBS for 3×3 minutes.

[0056] (5) Block with normal animal serum for 10 minutes, shake off excess serum without washing, add CD34 and β-Tubulin-Ⅲ mixed prima...

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Abstract

The invention belongs to the technical field of immunohistochemistry and relates to a double-labeled immunohistochemical staining kit for micro invasive lung adenocarcinoma. In order to solve the problem that invasive components and non-invasive components of micro invasive lung adenocarcinoma in HE staining films are difficultly distinguished clearly, the CD34 and beta-Tubulin-III double-labeled immunohistochemical staining kit is prepared and is applied to identification of the invasive lesions and the non-invasive components in the early stage of the lung adenocarcinoma, and overdiagnosis or under-diagnosis of the lung adenocarcinoma, caused by artificial interpretation difference, can be avoided.

Description

technical field [0001] The invention belongs to the technical field of immunohistochemistry, and particularly relates to a double-labeled immunohistochemical staining kit for displaying early infiltrating foci of microinvasive lung adenocarcinoma. technical background [0002] The treatment strategies for different stages of lung adenocarcinoma are different, so its pathological classification is of great significance for clinical treatment and prognosis. In the 2011 IASLC / ATS / ERS classification of lung adenocarcinoma, the morphological characteristics of lung adenocarcinoma stained by HE staining were mainly used for classification. However, these diagnostic criteria often encounter some problems in practical application, for example, there is no clear boundary between the collapsed lepidic adenocarcinoma and the infiltrating alveolar adenocarcinoma; lepidic adenocarcinoma grows in focal clusters , Simple papillary, papillary adenocarcinoma, micropapillary adenocarcinoma m...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/531
CPCG01N33/57407G01N2800/12
Inventor 杨清海郑智勇
Owner FUZHOU MAIXIN BIOTECH CO LTD
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