Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Double-labeled immunohistochemical staining kit for micro invasive lung adenocarcinoma

A technique for immunohistochemistry and staining reagents, which is used in measuring devices, instruments, disease diagnosis, etc., to achieve the effects of short experiment time, fewer staining steps, and intuitive comparison of experimental results.

Active Publication Date: 2014-04-23
FUZHOU MAIXIN BIOTECH CO LTD
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on our findings, we designed a CD34 and β-Tubulin-Ⅲ double-labeled immunohistochemical staining kit for displaying the invasive and non-invasive components of microinvasive lung adenocarcinoma on one section to avoid human interpretation Overdiagnosis or underdiagnosis of minimally invasive lung adenocarcinoma due to differences

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Double-labeled immunohistochemical staining kit for micro invasive lung adenocarcinoma
  • Double-labeled immunohistochemical staining kit for micro invasive lung adenocarcinoma
  • Double-labeled immunohistochemical staining kit for micro invasive lung adenocarcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The research objects were formaldehyde-fixed-paraffin-embedded microinvasive lung adenocarcinoma tissues (Department of Pathology, Fuzhou General Hospital of Nanjing Military Region). The steps of immunohistochemical experiment are as follows:

[0028] (1) The tissue slices were dry-baked in a 67°C incubator for 2 hours.

[0029] (2) Conventional xylene dewaxing 3 times, 6 minutes each time, hydration in 100%, 100%, 95%, 85% gradient ethanol, 3 minutes each time, and finally rinsed with tap water.

[0030] (3) High-pressure repair in citric acid antigen retrieval solution of pH 6.0 for 1.5-2 minutes, naturally cool to room temperature, rinse with tap water, and rinse with PBS for 3×3 minutes.

[0031] (4) Incubate in 3% hydrogen peroxide at room temperature for 10 minutes to block endogenous peroxidase, wash with PBS for 3×3 minutes.

[0032] (5) Block with normal animal serum for 10 minutes, shake off excess serum without washing, add CD34 and β-Tubulin-Ⅲ mixed prima...

Embodiment 2

[0039] The research objects were formaldehyde-fixed-paraffin-embedded microinvasive lung adenocarcinoma tissues (Department of Pathology, Fuzhou General Hospital of Nanjing Military Region). The steps of immunohistochemical experiment are as follows:

[0040] (1) The tissue slices were dry-baked in a 67°C incubator for 2 hours.

[0041] (2) Conventional xylene dewaxing 3 times, 6 minutes each time, hydration in 100%, 100%, 95%, 85% gradient ethanol, 3 minutes each time, and finally rinsed with tap water.

[0042] (3) High-pressure repair in citric acid antigen retrieval solution of pH 6.0 for 1.5-2 minutes, naturally cool to room temperature, rinse with tap water, and rinse with PBS for 3×3 minutes.

[0043] (4) Incubate in 3% hydrogen peroxide at room temperature for 10 minutes to block endogenous peroxidase, wash with PBS for 3×3 minutes.

[0044] (5) Block with normal animal serum for 10 minutes, shake off excess serum without washing, add CD34 and β-Tubulin-Ⅲ mixed prima...

Embodiment 3

[0051] The research objects were formaldehyde-fixed-paraffin-embedded microinvasive lung adenocarcinoma tissues (Department of Pathology, Fuzhou General Hospital of Nanjing Military Region). The steps of immunohistochemical experiment are as follows:

[0052] (1) The tissue slices were dry-baked in a 67°C incubator for 2 hours.

[0053] (2) Conventional xylene dewaxing 3 times, 6 minutes each time, hydration in 100%, 100%, 95%, 85% gradient ethanol, 3 minutes each time, and finally rinsed with tap water.

[0054] (3) High-pressure repair in citric acid antigen retrieval solution of pH 6.0 for 1.5-2 minutes, naturally cool to room temperature, rinse with tap water, and rinse with PBS for 3×3 minutes.

[0055] (4) Incubate in 3% hydrogen peroxide at room temperature for 10 minutes to block endogenous peroxidase, wash with PBS for 3×3 minutes.

[0056] (5) Block with normal animal serum for 10 minutes, shake off excess serum without washing, add CD34 and β-Tubulin-Ⅲ mixed prima...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of immunohistochemistry and relates to a double-labeled immunohistochemical staining kit for micro invasive lung adenocarcinoma. In order to solve the problem that invasive components and non-invasive components of micro invasive lung adenocarcinoma in HE staining films are difficultly distinguished clearly, the CD34 and beta-Tubulin-III double-labeled immunohistochemical staining kit is prepared and is applied to identification of the invasive lesions and the non-invasive components in the early stage of the lung adenocarcinoma, and overdiagnosis or under-diagnosis of the lung adenocarcinoma, caused by artificial interpretation difference, can be avoided.

Description

technical field [0001] The invention belongs to the technical field of immunohistochemistry, and particularly relates to a double-labeled immunohistochemical staining kit for displaying early infiltrating foci of microinvasive lung adenocarcinoma. technical background [0002] The treatment strategies for different stages of lung adenocarcinoma are different, so its pathological classification is of great significance for clinical treatment and prognosis. In the 2011 IASLC / ATS / ERS classification of lung adenocarcinoma, the morphological characteristics of lung adenocarcinoma stained by HE staining were mainly used for classification. However, these diagnostic criteria often encounter some problems in practical application, for example, there is no clear boundary between the collapsed lepidic adenocarcinoma and the infiltrating alveolar adenocarcinoma; lepidic adenocarcinoma grows in focal clusters , Simple papillary, papillary adenocarcinoma, micropapillary adenocarcinoma m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/574G01N33/531
CPCG01N33/57407G01N2800/12
Inventor 杨清海郑智勇
Owner FUZHOU MAIXIN BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com