Inoculation device and inoculation method

Abstract

The invention relates to an inoculation device, provides an inoculation method realized by the aid of the device simultaneously, and belongs to the technical field of pesticide biological assay for plant protection. The device comprises a vertical cylindrical tower, wherein a lifting mesh cage is suspended in the center of the top of the tower; at least three upwards inclined pneumatic nozzles are uniformly and circumferentially distributed on the side wall of the lower part of the tower; spraying spread angles of the pneumatic nozzles form an airflow convergence zone which can contain the mesh cage; and a drawer is mounted at the bottom of the tower. According to the device, conidial powder can be blown and separated from attacked leaves once by the aid of vertical airflow, separated conidial powder is floated uniformly, rotation cannot be caused when the conidial powder is blown, and obstruction is free when the conidial powder falls, so that the conidial powder can uniformly fall and spread on a host plant sample plate with a larger area, and biological assay and assessment with high identity and repeatability are realized.

Description

technical field [0001] The invention relates to an inoculation device, in particular to an inoculation device for uniform powder spraying, and at the same time provides an inoculation method realized by the device, belonging to the technical field of pesticide bioassay for plant protection. Background technique [0002] "Inoculation" in the field of pesticide bioassay refers to artificially bringing pathogens into contact with susceptible parts of host plants, so as to create conditions for pathogens to invade and induce host plant disease. Inoculation is an important step in the process of syndrome and disease. "Inoculation" is indispensable for studying the pathogenesis of parasitism, measuring the disease resistance of varieties, and the effect of pesticides on disease prevention. [0003] As far as the applicant knows, commonly used inoculation methods include smearing and spraying. The disadvantage of the smear method is that it cannot be done uniformly every time, and...

AI Technical Summary

Problems solved by technology

The disadvantage of the smear method is that it cannot be done uniformly every time, and the spray method changes the transmission mode of the spore powder for airborne bacteria, and it is difficult to make a correct assessment

In addition, there is a method of directly shaking the spore powder of diseased leaves onto different host plant samples artificially, which makes it difficult to control the number of deposited spores on each sample to be basically the same

[0004] People have designed special-purpose inoculation device for this reason, to realize the uniform settlement of germ spore powder, for example " Jiangsu Agricultural Science " 1986 05 issues have disclosed " powdery mildew simple inoculation tower ", and its tower barrel middle part is equipped with the electric fan that blows upwards. The fan is equipped with a rotatable storage table at the bottom. When in use, the diseased leaves are shaken from the tower mouth at the top, so that they are blown away and then fall to the host plant model on the storage table. The disadvantage is that the airflow blown upward by the electric fan A rotating airflow moving along a circle will be formed, and the spore powder rotating with the airflow tends to settle along the peripheral wall of the tower body, resulting in more sedimentation spore powder around the storage table, and the distribution of sedimentation along the radial direction is very uneven, and the electric fan blocks the central part. drop of spore meal

[0005] As another example, "The American Phytopathological Society" Vol78, No11, 1988-1463 of the U.S. discloses "A Vacuum-Operation Settling Tower for Inoculation of Powdery Mildew Fungi. Powdery Mildew Fungi Inoculation Vacuum Settling Tower", the top of the tower has a sealed The cover has a small cavity for diseased leaves, and a storage table is installed at the bottom. After vacuuming, the sealing cover is opened, and the airflow caused by negative pressure can be used to scatter the germ spore powder onto the host plant model on the storage table. The disadvantage is that it depends on the sealing performance. The negative-pressure airflow formed by vacuuming the limited cavity is difficult to blow off the spore powder on the diseased leaves at one time, and this negative-pressure airflow is only formed at one time when the sealing cover is opened, and the downward airflow is difficult to blow the spore powder away. The powder evenly settles on the sample with a larger area

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