A kind of preparation method of specific nano gold dna probe

Abstract

The invention discloses a preparation method of a specific nano gold DNA probe. The method includes two steps of preparing 13nm gold nanoparticle AuNPs and connecting a specific probe to the 13nm gold nanoparticle AuNPs. The specific probe includes the complementary sequence of the 16SrRNA conserved sequence and the complementary sequence sandwiching the complementary sequence of the 16SrRNA conserved sequence. The two complementary hairpin sequences of 16SrRNA, and the fluorescent molecule FAM and its quenching group BHQ1 sandwiching the complementary sequence of the 16SrRNA conserved sequence and the two complementary hairpin sequences. Compared with the traditional bacterial detection method of urine samples, the specific nano-gold DNA probe prepared by this method has more significant, rapid, sensitive and specific advantages when detecting urine samples.

Description

technical field [0001] The invention relates to the field of nucleic acid detection, in particular to a method for preparing a specific nano gold DNA probe. Background technique [0002] Bacterial infection is a widespread disease in the world, such as pneumonia, tuberculosis, urinary tract infection and other diseases are caused by bacteria, which has brought great trouble to the normal work and study of human beings. Among them, bacterial urinary tract infection is the second most common bacterial infection of the tissue system. Approximately seven million patients are affected each year, and more than one million require hospitalization. The annual cost of treating the disease is huge, which has a great impact on the country's economic development and social productivity, and a large part of the cost is used for bacterial detection, but the traditional bacterial detection of urine samples is the most important The disadvantage is that it takes a long time. It takes abou...

AI Technical Summary

Problems solved by technology

The annual cost of treating the disease is huge, which has a great impact on the country's economic development and social productivity, and a large part of the cost is used for bacterial detection, but the traditional bacterial detection of urine samples is the most important The disadvantage is that it takes a long time. It takes about two days from sample collection to test results. Cultivating cloned bacteria is the main time-consuming process.

In clinical practice, because the bacterial test results are not obtained in time, the administration is only based on experience, and adverse reactions and bacterial drug resistance caused by improper use of drugs often occur clinically.

At present, the developed molecular detection technology and the application of PCR technology have certain advantages, but both need to amplify the target sequence, which is not convenient enough (1, Journal of clinical microbiology2009, 47(8), 2405-10.2, Journal of clinical microbiology2009 , 47(7), 2067-78.)

For western blotting, the required steps are more cumbersome (3, Genome biology2007, 8(5), R93.)

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