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Artificial disease inducing method by inoculating tobacco damping off on in-vitro leaf vein

A technology for leaf veins and blight, applied in the fields of agricultural technology and biology, to achieve high work efficiency, good reproducibility, and easy control

Active Publication Date: 2014-05-14
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the disease mainly harms the stem base of tobacco plants, the inventors have found that R. solani can also infect the upper leaves of tobacco and cause clear necrotic lesions. This pathogenic characteristic can be used in artificial inoculation. Tobacco blight can infect the favorable characteristics of leaves and cause disease, apply it in the practice of artificial disease and establish supporting technologies

Method used

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  • Artificial disease inducing method by inoculating tobacco damping off on in-vitro leaf vein
  • Artificial disease inducing method by inoculating tobacco damping off on in-vitro leaf vein
  • Artificial disease inducing method by inoculating tobacco damping off on in-vitro leaf vein

Examples

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Effect test

Embodiment 1

[0037] An artificial pathogenic method for inoculating tobacco blight by using isolated leaf veins, inoculating the tobacco seedling variety Yunyan 87 on the in vitro leaves of tobacco blight bacilli strain Rs-1, according to the following steps:

[0038] 1. Preparation of utensils for inoculation: utensils for inoculation include, such as Figure 4 The large square plate a, the small square plate b, the glass cover c, the working panel d, the support plate e and the support sheet f shown in the figure, the material and size of the appliance in this embodiment: the large square plate a is length × width × height = 40 × 30 × 3cm ordinary square plate; small square plate b is an ordinary square plate with length × width × height = 29 × 22 × 3cm; glass cover c is a plexiglass cover with length × width × height = 31 × 25 × 20cm; working panel d is a common glass plate of 24 × 24cm, the support plate e is a small glass plate of 24 × 9cm; Wash these components with detergent before...

Embodiment 2

[0049] An artificial pathogenic method for inoculating tobacco blight by using isolated leaf veins, inoculating tobacco blight strain Rs-1 on the isolated leaves of tobacco seedling variety Yunyan 87, and performing operations according to steps 1 to 7 of Example 1, The operation in Step 7 is different from the operation in Step 7 of Example 1. In Step 7 of Example 1, the culture temperature after inoculation was maintained at 26-28°C. In Step 7 of this embodiment, the culture temperature after inoculation was maintained at 20°C. ~22°C. As a result, the isolated leaf vein slices also showed the following figure 2 and image 3 The same clear necrotic lesions, but the speed of lesion expansion is slower than that of Example 1, and the widest distance of necrotic lesions is 5.9cm in 120 hours after inoculation.

Embodiment 3

[0051] An artificial pathogenic method for inoculating tobacco blight by using isolated leaf veins, inoculating tobacco blight strain Rs-1 on the isolated leaves of tobacco seedling variety Yunyan 87, and operating according to step 1) to step 7) of Example 1 Implementation, wherein the operation in step 3) is different from the operation in step 3) of Example 1. The cultivation time of step 3) of the inoculum mycelia in Example 1 is 48 hours. The step 3) of the inoculum in this example The silk was cultured for 96 hours. As a result, the isolated leaf vein slices also showed the following figure 2 and image 3 The same clear necrotic lesions, but the speed of lesion expansion is slower than that of Example 1, and the widest distance of necrotic lesions in 72 hours after inoculation is 2.8cm.

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Abstract

The invention discloses an artificial disease inducing method by inoculating tobacco damping off on in-vitro leaf veins. The method comprises the following steps: 1) preparing tools for inoculation, 2) culturing tobacco seedlings, 3) preparing inoculation mycelium, 4) collecting and treating in-vitro leaf veins, 5) putting the in-vitro leaf veins on the tools for inoculation, 6) inoculating germs, and 7) performing later treatment after inoculation. The method has the beneficial effects that firstly, the inoculation disease induction time is short, a small space is occupied, the method is simple, easy, rapid and convenient, and the working efficiency is high; secondly, large tobacco leaves are used in the method, so that the difference of result disease situations can be easily reflected, the obtaining of the tobacco leaves is ensured, the disease symptoms are clear and easy to identify, the reproducibility is good, the partial conditions for inoculation disease induction are easy to control, and the standardization of the technical method is easy to realize.

Description

technical field [0001] The present invention relates to agricultural technology and biotechnology. Specifically, it is an artificial pathogenic method for inoculating tobacco blight with isolated leaf veins. Background technique [0002] In the study of plant disease control and prevention, the research on many basic issues or important theoretical issues cannot be separated from the basic means of artificial inoculation. The artificial disease of plant diseases can be inoculated on living plants, and can also be inoculated on isolated plant organ materials. Although the research results of using living plants to inoculate the pathogenesis can better reflect the original appearance of the host-bacteria interaction, inoculation on living organisms often requires more time and space, and consumes more energy and financial resources. However, it is difficult to control local conditions; while the use of isolated plant organ materials to inoculate the disease often requires le...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 张君成李界秋黄柳陈江凝谭金乐
Owner GUANGXI UNIV