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Aspergillus niger capable of efficiently degrading nicosulfuron and application of aspergillus niger

A technology of Aspergillus niger and Aspergillus niger, which is applied to the application field of Aspergillus niger in the remediation of soil contaminated by sulfonylurea herbicides, and can solve the problems of great differences in sensitivity of different crops and phytotoxicity of sensitive crops after cropping.

Active Publication Date: 2014-05-21
HEBEI TIANFA CHEM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of herbicide has the advantages of strong selectivity and high biological activity, but its sensitivity to different crops varies greatly. Harmful

Method used

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  • Aspergillus niger capable of efficiently degrading nicosulfuron and application of aspergillus niger
  • Aspergillus niger capable of efficiently degrading nicosulfuron and application of aspergillus niger

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 The separation of Aspergillus niger bacterial strain of the present invention

[0020] 1, Domestication treatment and enrichment culture of microorganisms in water samples

[0021] Add nicosulfuron-methyl to the test water sample to make the content 400 mg·L -1 , put it at 30°C, 150 r·min -1 14 days of acclimatization and cultivation under the same conditions, 5 mL of acclimatized water samples were added to 50 mL of 200 mg·L -1 Nicosulfuron enriched medium, placed at 30°C, 150 r min -1 Shaking culture under the conditions for 7 days, then take 5 mL of culture solution, transfer to 50 mL containing 200 mg·L -1 Shaking culture was carried out in the rich medium of nicosulfuron, and this was repeated for a total of 5 times.

[0022] 2, Screening of microbial strains capable of degrading nicosulfuron

[0023] Spread the above-mentioned acclimatized culture medium containing water-like microorganisms evenly to contain 400 mg·L -1 On the PDA plate of ...

Embodiment 2

[0025] Embodiment 2 Identification of Aspergillus niger bacterial strain of the present invention

[0026] 1 Morphological identification

[0027] According to the "Fungus Identification Manual" and other methods, the PDA medium was used to cultivate the fungi, the growth characteristics of the colony were observed with the naked eye, the growth rate of the colony was measured, and the shape of the hyphae and the size, shape, and type of the spores were observed under an optical microscope. Through observation, it is found that it protrudes from the middle, with a diameter of 15-20pm, a length of about 1-3mm, and a thick and smooth wall. A spherical apical capsule is formed on the top, which is fully covered with a layer of stem base and a layer of small stems. On the small stems, there are clusters of brown-black spherical shapes with a diameter of 2.5-4.0 μm. Conidia heads spherical, 700-800 μm in diameter, brown-black. It spreads rapidly and is white at first, then turn...

Embodiment 3

[0030] Embodiment 3 Aspergillus niger of the present invention is to the degradation efficiency of nicosulfuron-methyl

[0031] Put nicosulfuron in the PDA medium to make the final concentration 400 mg·L-1, and prepare a PDA plate. After the medium is completely solidified, take the plate of Aspergillus niger and place it on the nicosulfuron Long the center of the PDA plate, place it in a 37°C incubator and culture it for 1 day, then detect the presence and size of the degradation circle.

[0032] Repeat the test, the basic method is the same as above, 3 strains are spotted on each plate, and each is repeated 3 times.

[0033] After testing, it was found that Aspergillus niger of the present invention had a strong degradation effect on nicosulfuron, and a transparent degradation circle was formed after 24 hours. The diameter of the degradation circle was 3.8 mm, as shown in Table 1.

[0034] Table 1 Degradation cycle of nicosulfuron by Aspergillus niger

[0035]

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Abstract

The invention relates to aspergillus niger capable of efficiently degrading nicosulfuron, and application of the aspergillus niger on soil remediation. The aspergillus niger is preserved in the China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC NO.8438. 400mg / L<-1> of nicosulfuron can be completely degraded by the aspergillus niger within five days, the aspergillus niger does not pollute the environment, and is ecologically safe, thus being a strain having high application value on the remediation of soil polluted by sulfonylurea herbicides, and having practical application meaning and value.

Description

technical field [0001] The invention relates to the field of microorganisms and environmental protection, in particular to the application of Aspergillus niger in remediation of soil polluted by sulfonylurea herbicides. [0002] technical background [0003] Sulfonylurea herbicides are currently the most widely used herbicides in the world. It was successfully researched by DuPont in the 1970s, and the wheat field herbicide chlorsulfuron was first developed in 1982, and later developed Many high-efficiency, broad-spectrum, low-toxicity, high-selectivity sulfonylurea herbicides have been produced. These herbicides act on the plant's acetolactate synthase (ALS), resulting in the inability to synthesize branched-chain amino acids (valine, leucine, and isoleucine), resulting in cellular inhibition and are effective against many annual or perennial weeds It has special effects and is widely used to control weeds in rice fields, soybean fields, corn fields, wheat crop fields, ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10C05G3/00C12R1/685
Inventor 赵斌张金林董金皋邢继红霍静倩
Owner HEBEI TIANFA CHEM TECH CO LTD
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