Nanometer adjuvant and preparation method for same

A kind of adjuvant, nano technology, applied in the field of nano adjuvant and its preparation

Inactive Publication Date: 2014-05-28
乾元浩生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The use of nanoemulsions in drugs has been reported for oral and external use, but there are fewer reports in the field of animal vaccines, while nanoadjuvant vaccines are more researched on nanoparticles, such as aluminum hydroxide nanoparticles, calcium phosphate nanoparticles and PLA and chitosan materials with better biocompatibility, etc.

Method used

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  • Nanometer adjuvant and preparation method for same
  • Nanometer adjuvant and preparation method for same
  • Nanometer adjuvant and preparation method for same

Examples

Experimental program
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Effect test

Embodiment 1

[0042] The preparation of embodiment 1 nano bird flu vaccine

[0043]The components weighed are as follows: mineral oil for injection MARCOL52 is 95%, lipophilic emulsifier sucrose fatty acid ester is 3%, sorbitan monooleate is 1%; hydrophilic emulsifier polyoxyethylene sorbitan monooleate 0.5% ester, 0.5% polyoxyethylene (20) oleyl ether.

[0044] The nano adjuvant was prepared according to the above nano adjuvant production process, after being sterilized at 121°C for 30 minutes and cooled, 1000ml was added to the emulsification tank of the shearing machine. According to the standard, 300ml of chicken embryo inactivated avian influenza antigen H5N1 (Re-6) was slowly added into the emulsification tank, sheared and emulsified for 3 minutes, and then sheared and emulsified for 5 minutes under the condition of 8000 rpm to make a nano-sized avian influenza vaccine. To be detected after being marked. Physical indicator detection and animal experiments were carried out after 24 h...

Embodiment 2

[0047] The preparation of embodiment 2 nanometer bird flu vaccines

[0048] Weigh the components as follows: 90% mineral oil MARCOL52 for injection, 0.5% lipophilic emulsifier sucrose fatty acid ester, 0.5% sorbitan monooleate; hydrophilic emulsifier polyoxyethylene (20) oleyl ether 3 %, Pluronic F68 is 1%, additives polyethylene glycol 200 is 3%, polyethylene glycol 800 is 1%, stearic acid 0.5%, palmitic acid 0.5%.

[0049] The above raw materials are made into nano-adjuvant according to the above-mentioned production process of nano-adjuvant. After sterilizing at 121°C for 30 minutes and cooling, take 1000ml and add it to the emulsification tank of the shearing machine. Slowly add 200ml of chicken Newcastle disease (ZM10 strain) inactivated antigen that meets the standards for production into the emulsification tank, shear and emulsify for 3 minutes, and then shear and emulsify for 5 minutes under the condition of 8000 rpm to make a nano-sized bird flu vaccine. Marked to be...

Embodiment 3

[0052] The preparation of embodiment 3 nano bird flu vaccines

[0053] Mineral oil for injection is Hangzhou Petrochemical injection grade white oil 40%, lipophilic emulsifier sorbitol trioleate 13%, sucrose fatty acid ester 12%; hydrophilic emulsifier polyoxyethylene (23) lauryl ether 11% , polyoxyethylene sucrose fatty acid ester is 14%, additive polyethylene glycol 400 is 6%, stearyl alcohol is 3%, and palmitic acid is 1%.

[0054] The above raw materials are made into nano-adjuvant according to the above-mentioned nano-adjuvant animal vaccine production process. After sterilizing at 121°C for 30 minutes and cooling, take 1000ml and add it to the emulsification tank of the shearing machine. At 25°C, the shear rate is 1000 rpm Slowly add 200ml antigen of chicken Newcastle disease and avian influenza double-inactivated vaccine (LaSota strain + F strain) that meets the standards for production into the emulsification tank, shear and emulsify for 3 minutes, and then shear and e...

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Abstract

The invention relates to a nanometer adjuvant and a preparation method for the same. The nanometer adjuvant comprises the following components in percentage by weight: 40 to 95 percent of injection mineral oil, 1 to 25 percent of oleophilic emulsifiers, 0 to 25 percent of hydrophilic emulsifiers and 0 to 10 percent of auxiliaries. A nanometer adjuvant animal vaccine prepared from the adjuvant is a novel stable, safe and efficient clinical animal vaccine.

Description

technical field [0001] The invention relates to a vaccine adjuvant, in particular to a nano adjuvant and a preparation method thereof. Background technique [0002] An adjuvant refers to a substance that can be applied simultaneously or in advance with an antigen to enhance the body's immune response to the antigen, or to change the type of immune response. Adjuvants play a vital role in vaccines. They can act on both antigens and the body. Appropriate use of adjuvants can regulate or even change the type of antigen-specific humoral and / or cellular immune responses produced by the body's immune system. [0003] Immunopharmacology studies have shown that the same antigen, using immune adjuvants with different physical and chemical properties, changing the way and method of immunization, designing different vaccine and adjuvant delivery systems, carriers and formulations, different combinations of antigens and adjuvants, using different antigens Epitopes, etc., it is entirely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/145A61K39/17A61K39/135A61K39/12A61K39/205A61P37/04A61P31/16A61P31/14
Inventor 潘京学刘兆环王贺民潘春刚严石
Owner 乾元浩生物股份有限公司
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