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Ginsenoside Rg1-phenyl isocyanate chiral stationary phase filler and preparation method thereof

A technology of phenyl isocyanate and chiral stationary phase is applied in the field of analytical chemistry to achieve the effect that the preparation method is simple and meets the needs of daily drug analysis and production quality control

Inactive Publication Date: 2015-07-22
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phenyl isocyanate derivatives of ginsenoside Rg1 chiral stationary phase are used as chiral selective agents for chromatographic separation, but there is no report at home and abroad

Method used

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  • Ginsenoside Rg1-phenyl isocyanate chiral stationary phase filler and preparation method thereof
  • Ginsenoside Rg1-phenyl isocyanate chiral stationary phase filler and preparation method thereof
  • Ginsenoside Rg1-phenyl isocyanate chiral stationary phase filler and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] In this example, the natural product-ginsenoside Rg1 is used as a raw material, chemically bonded to a silica gel carrier silanized with 3-(aminopropyl)-triethoxysilane through a spacer arm, and then derivatized with phenylisocyanate to prepare to make.

[0030] The specific separation parameters of the amino acid in the reversed-phase mobile phase are as follows:

[0031] The mobile phase was acetonitrile / 0.05% glacial acetic acid-triethylamine buffer salt=90 / 10. The flow rate is 0.4mL / min, the separation results are shown in the table below and Figures 1 to 9 ; The detection wavelength is 210nm.

[0032] The table shows the separation results of ginsenoside Rg1-phenylisocyanate chiral immobilization relative amino acid enantiomers in the reversed-phase mobile phase:

[0033]

[0034]

[0035] Mobile phase: acetonitrile / 0.05% glacial acetic acid-triethylamine buffer salt=90 / 10. The flow rate is 0.4mL / min, and the detection wavelength is 210nm.

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Abstract

The invention discloses a ginsenoside Rg1-phenyl isocyanate chiral stationary phase filler and a preparation method thereof, and relates to the technical field of analytical chemistry; the method comprises the following steps: chemically bonding a natural product, ginsenoside Rg1, onto a silica-gel carrier, and then carrying out derivatization reaction with phenyl isocyanate. By using the ginsenoside Rg1-phenyl isocyanate as a chiral chromatography stationary phase filler, the ginsenoside Rg1-phenyl isocyanate chiral stationary phase filler has a chiral recognition capability and excellent stability performance, can be used in a condition of reversion phase chromatography, and has a certain separating capability to amino acid.

Description

Technical field: [0001] The invention relates to a ginsenoside Rg1-phenylisocyanate chiral stationary phase filler and a preparation method thereof, belonging to the technical field of analytical chemistry. Background technique: [0002] Chirality is one of the essential attributes of nature on which human beings depend. Biomacromolecules such as proteins, polysaccharides, and nucleic acids all have chirality. Optically active chiral substances widely exist in animals and plants. This biological stereoselectivity and stereosynthesis of optically active substances are unique instincts of biological systems. Two chiral compounds that are enantiomers often have large differences in biological activity, pharmacokinetics, and potential side effects of drugs. About half of the chemical drugs currently on the market contain chiral centers. , about half of which are sold in the form of racemates. For this reason, the health management departments of the United States, the European ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/29B01J20/30G01N30/02
Inventor 沈报春郭伟
Owner KUNMING MEDICAL UNIVERSITY