Fungus PcGDH protein for improving efficient utilization of nitrogen, and application thereof

A high-efficiency, protein-based technology, applied in the field of plant genetic engineering, can solve problems such as increased photosynthetic efficiency and reduced dark respiration efficiency, and achieve the effects of improving utilization, increasing yield, and reducing environmental pollution

Inactive Publication Date: 2014-05-28
HUNAN UNIV +1
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Problems solved by technology

The study found that compared with untransformed control plants, transgenic potatoes had increased leaf area and photosynthetic efficiency under both standard nitrogen and low nitrogen conditions, but dark respiration efficiency was greatly reduced

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  • Fungus PcGDH protein for improving efficient utilization of nitrogen, and application thereof
  • Fungus PcGDH protein for improving efficient utilization of nitrogen, and application thereof
  • Fungus PcGDH protein for improving efficient utilization of nitrogen, and application thereof

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with the accompanying drawings and embodiments. The experimental methods mentioned in the following examples are conventional methods unless otherwise specified.

[0031] PcGDH gene cloning

[0032] Find the conserved region of the GDH gene from different fungal sources through the sequence of the GDH gene of the fungal strains that have been sequenced so far, and use primer5 to design degenerate primers to amplify the fungus Pleurotus cystidiosus GDH gene. The forward degenerate primer starts from the start codon of the open reading frame, the sequence is 5'-ATGTCCCACCTGCCTTTCGARCCNGARTT-3', and the reverse degenerate primer is located behind the 3' end of the open reading frame, the sequence is 5'-CCCCACCAGTCACCCCTGGTCRTGCATNGC-3'; then the fungus Pleurotus cystidiosus The cDNA was used as a template and cloned by RT-PCR method PcGDH Gene. According to the method of Chen et al (2006) the ...

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Abstract

The invention belongs to the field of plant genetic engineering, and discloses clone and application of a fungus PcGDH (Pleurotuscystidiosus Glutamate Dehydrogenase) gene for improving efficient utilization of nitrogen. The NADP (H) (Nicotinamide Adenine Dinucleotide Phosphate) enzymatic activity of a PcGDH in-vitro positive reaction is larger than that of a reverse reaction, that is the PcGDH protein is inclined to utilize NH4+ to translate alpha-oxoglutarate into glutamic acid. In addition, the affinity of the PcGDH to NH4+ is larger than glutamic acid. By virtue of a genetic engineering technology, the PcGDH gene is over-expressed in rice in a heterogeneous manner to improve utilization of nitrogen by transgenic rice, improve growth of the transgenic rice and increase absolute plant and thousand seed weight of the transgenic rice. Therefore, the PcGDH gene for improving the rice nitrogen can be utilized to culture novel rice products with good agronomic characters.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, the present invention relates to rice ( Oryza sativa Heterologously expressed fungi in L.) Pleurotus cystidiosus glutamate dehydrogenase gene PcGDH , can improve the efficient use of nitrogen in rice and improve the growth of rice. Background technique [0002] Rice is one of the food crops with the largest planting area, the highest yield, and the largest amount of nitrogen fertilizer in my country. The impact of nitrogen (N) on rice production is second only to water, and it constitutes the main part of rice production cost. According to the statistics of the World Food and Agriculture Organization (FAO 2001), the average annual rice planting area in China from 1995 to 1997 was 31.7×10 6 hm 2 , accounting for 20% of the world's rice planting area, and rice nitrogen fertilizer use accounts for 37% of the world's total rice nitrogen fertilizer use, becoming the coun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/04C12N15/53C12N15/84A01H5/00
CPCC12N9/0016C12N15/8243C12Y104/01004
Inventor 刘选明刘泓周延彪邓小龙林建中王宗华唐冬英
Owner HUNAN UNIV
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