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Liver cancer patient source heterotransplantation tumour mouse model and construction method thereof

A technology of xenotransplantation and mouse model, which is applied in animal husbandry and other fields, achieves the effect of low mortality rate, simple and easy establishment process, and easy promotion

Active Publication Date: 2014-06-04
南京普恩瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The planting success rate of this method is only 20.83%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A liver cancer patient-derived xenograft tumor mouse model consists of mice subcutaneously inoculated with treated liver cancer tissue blocks on the shoulder and back of the forelimb, and the establishment method is as follows:

[0020] (1) Preparation of nutrient solution: 90 parts by volume of DMEM are mixed with 10 parts by volume of fetal bovine serum, and penicillin and streptomycin are added to obtain DMEM nutrient solution, wherein the final concentration of penicillin is 100 U / ml, and the concentration of streptomycin The final concentration was 100 U / ml to obtain a culture solution.

[0021] (2) Prepare sterile incubation solution: mix 45 parts by volume of DMEM, 5 parts by volume of fetal calf serum and 50 parts by volume of Matrigel glue (BD Company; item number: 356234), add epidermal growth factor (EGF) (Gibco Company ; product number: PHG0314), fibroblast growth factor (bFGF) (Gibco company; product number: PHG0264), penicillin and streptomycin, obtain ste...

Embodiment 2

[0028] A liver cancer patient-derived xenograft tumor mouse model consists of mice subcutaneously inoculated with treated liver cancer tissue blocks on the shoulder and back of the forelimb, and the establishment method is as follows:

[0029] (1) Preparation of nutrient solution: 90 parts by volume of DMEM are mixed with 10 parts by volume of fetal bovine serum, and penicillin and streptomycin are added to obtain DMEM nutrient solution, wherein the final concentration of penicillin is 100 U / ml, and the concentration of streptomycin The final concentration was 100 U / ml to obtain a culture solution.

[0030](2) Prepare sterile incubation solution: mix 45 parts by volume of DMEM, 5 parts by volume of fetal calf serum and 50 parts by volume of Matrigel glue (BD Company; item number: 356234), add epidermal growth factor (EGF) (Gibco Company ; product number: PHG0314), fibroblast growth factor (bFGF) (Gibco company; product number: PHG0264), penicillin and streptomycin, obtain ster...

Embodiment 3

[0037] A liver cancer patient-derived xenograft tumor mouse model consists of mice subcutaneously inoculated with treated liver cancer tissue blocks on the shoulder and back of the forelimb, and the establishment method is as follows:

[0038] (1) Preparation of nutrient solution: 90 parts by volume of DMEM are mixed with 10 parts by volume of fetal bovine serum, and penicillin and streptomycin are added to obtain DMEM nutrient solution, wherein the final concentration of penicillin is 100 U / ml, and the concentration of streptomycin The final concentration was 100 U / ml to obtain a culture solution.

[0039] (2) Prepare sterile incubation solution: mix 45 parts by volume of DMEM, 5 parts by volume of fetal calf serum and 50 parts by volume of Matrigel glue (BD Company; item number: 356234), add epidermal growth factor (EGF) (Gibco Company ; product number: PHG0314), fibroblast growth factor (bFGF) (Gibco company; product number: PHG0264), penicillin and streptomycin, obtain ste...

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Abstract

The invention provides a liver cancer patient source heterotransplantation tumour mouse model and a construction method of the mouse model. The liver cancer patient source heterotransplantation tumour mouse model comprises a mouse, wherein treated liver tissue blocks are vaccinated under the skin of the fore limb, the shoulders and the back of the mouse. The construction method comprises the steps of taking fresh liver cancer tissue, processing the tissue, vaccinating the treated liver cancer tissue blocks under the skin below the fore limb, the shoulders and the back of the mouse without the tumor, conducting conventional breeding, and obtaining the liver cancer patient source heterotransplantation tumour mouse model. The construction process is simple and easy, the tumor forming occurrence rate reaches 40 percent to 50 percent, and the method can be used for liver cancer medicine screening and liver cancer mechanism research. The built liver cancer patient source heterotransplantation tumour mouse model has the advantages of being simple and easy to manufacture, low in death rate caused by operation, high in success rate, easy to popularize, capable of being manufactured in mass, and good in synchronism, and is suitable for medicine screening and experiment research.

Description

technical field [0001] The invention relates to a liver cancer patient-derived xenograft tumor mouse model and a construction method thereof. Background technique [0002] Primary liver cancer is the fifth malignant tumor in the world and the third in my country. According to the World Health Organization, before 2030, liver cancer will continue to grow, and its case fatality rate is the second highest. At present, surgery is still one of the main treatment methods for liver cancer, but more than 60% of liver cancer patients have entered the middle and advanced stages when they first see a doctor, accompanied by metastasis and recurrence, thus losing the opportunity for surgical treatment. [0003] Tumor animal models are an important platform for research on tumor-related mechanisms and drug screening. At present, preclinical tumor animal models mostly adopt the method of tumor cell line suspension modeling, which has the advantages of easy acquisition of tumor cell lines...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
Inventor 樊嘉杨欣荣胡博徐泱周俭
Owner 南京普恩瑞生物科技有限公司
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