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Method of preparing high-purity heparin sodium

A heparin sodium, high-purity technology, applied in the biological field, can solve the problems of complicated operation, time-consuming and labor-consuming, low yield, etc., and achieve the effect of simple and easy method

Inactive Publication Date: 2014-06-18
QINGDAO JIULONG BIO PHARMA
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  • Abstract
  • Description
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Problems solved by technology

[0003] In the existing separation technology, there is an ion exchange resin method for the separation of heparin sodium and similar substances CS and DS, and its disadvantages are: time-consuming, labor-intensive, reagent-consuming, complicated operation, and low yield
[0004] In view of the shortcomings of the above-mentioned separation technology, in order to solve the problems of the existing ion exchange resin method, such as reagent consumption, time-consuming and labor-consuming, complicated operation, etc., the present invention provides a method capable of rapidly separating heparin sodium and its similar substances and preparing high-purity heparin sodium. The method, the method of the present invention is green and pollution-free, simple and easy to implement, can effectively separate heparin sodium and its similar substances, and prepare high-purity heparin sodium

Method used

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  • Method of preparing high-purity heparin sodium

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Experimental program
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Effect test

Embodiment 1

[0019] (1) Weigh 5g of heparin industrial crude product (containing different amounts of DS, CS impurities);

[0020] (2) Add 2% salt water and dissolve evenly;

[0021] (3) adding an organic solvent to the solution with a volume multiple of 9.1%-28.6% (Volume);

[0022] (4) Oscillate evenly, and stand at room temperature for 2 hours;

[0023] (5) Centrifuge to collect the precipitate, then adjust the volume multiple of the supernatant to 33.3%-44.4% of the organic solvent, oscillate evenly, and store at room temperature;

[0024] (6) Centrifuge to collect the precipitate, adjust the volume multiple of the supernatant to 47.4%-66.7%, centrifuge, collect the precipitate, dry each part of the precipitate, and weigh.

[0025] (7) Table 1 shows the experimental results of ethanol fractional alcohol precipitation.

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Abstract

The invention discloses a method of preparing high-purity heparin sodium. The method is characterized in that under a room temperature of 298K, an organic precipitation method is adopted to remove heparin sodium-like impurities dermatan sulfate and chondroitin sulfate in the heparin sodium to prepare high-purity heparin sodium, so that the heparin sodium and similar substances thereof are separated according to solubility difference in different polar solvents. The method is suitable for purifying and separating the sodium-like impurities dermatan sulfate, chondroitin sulfate and the like in the heparin sodium to prepare the high-purity heparin sodium, and has remarkable social and beneficial benefits.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing high-purity heparin sodium. Background technique [0002] Heparin sodium and heparin-like substances chondroitin sulfate (CS) and dermatan sulfate (DS) belong to the mixture of mucopolysaccharide polymers. The extraction and separation of polysaccharides mostly adopts the process of water extraction and alcohol precipitation. The molecular weight is different, and the separation and purification are carried out, so when the organic solvent is precipitated, the products obtained with different volume fractions are different. According to the molecular weight of heparin sodium, CS, and DS, organic solvents are used to separate the impurity CS in heparin sodium through distribution precipitation. , DS. [0003] Among the existing separation technologies, there is an ion-exchange resin method for the separation of heparin sodium and similar substances CS and DS. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 刘乃山刘汝荣迟培升夏衬来
Owner QINGDAO JIULONG BIO PHARMA
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