Peanut peptide preparation method for enzymatically decomposing thermal-crushed peanut meal step by step with double neutral protease

Abstract

The invention discloses a peanut peptide preparation method for enzymatically decomposing thermal-crushed peanut meal step by step with double neutral protease. The method is characterized in that the thermal-crushed peanut meal is enzymatically decomposed through neutral protease and compound protease step by step, and experiments are carried out to determine the optimal enzymatic decomposing condition and the addition of the neutral protease and compound protease. The peanut peptide preparation method is simple in process, mild in condition, short in preparation time, low in preparation cost, low in yield of oligopeptide, and high in degree of hydrolysis, and the utilization rate of the thermal-crushed peanut meal is effectively raised. According to the method, the yield of oligopeptide and the degree of hydrolysis are greatly improved, the yield of oligopeptide reaches 85.20%, and the degree of hydrolysis reaches 40.55%; in addition, the molecular weight of the obtained peanut peptide is concentrated to be below 1000Da, and the peanut peptide with the molecular weight being less than 1000Da accounts for 95.57%, so that the prepared peanut peptide is easily absorbed and utilized by a human body; and the peanut peptide is high in functional activity and high in nutritional value. The method has the advantages of being simple in process, mild in condition, high in yield of oligopeptide, capable of producing peanut peptide with small molecular weight, and suitable for massive industrial production.

Description

technical field [0001] The invention relates to the field of peanut meal utilization, in particular to a method for preparing peanut peptides by step-by-step enzymolysis of hot-pressed peanut meal with dual neutral proteases. Background technique [0002] Peanut is an important oil crop in my country, and its output and export volume rank first in the world. Peanut protein contains eight essential amino acids, the arginine content is higher than other nuts, and the biological potency is higher than soybean. [0003] Peanut meal is a by-product of the peanut oil extraction process, with an annual output of about 3 million tons. According to different oil extraction processes, peanut meal is divided into hot-pressed peanut meal, solvent-extracted peanut meal and cold-pressed peanut meal. At present, my country’s peanut oil extraction mainly adopts hot pressing, and the content of peanut protein in hot-pressed peanut meal is 45% to 55%, but the degree of denaturation of peanut...

AI Technical Summary

Problems solved by technology

At present, my country’s peanut oil extraction mainly adopts hot pressing, and the content of peanut protein in hot-pressed peanut meal is 45% to 55%, but the degree of denaturation of peanut protein in hot-pressed peanut meal is serious, which affects its function and physiological activity. The yield of peanut protein is very low, so the current utilization of hot-pressed peanut meal is greatly restricted. Only less than 1% is used in the food industry, and most of them are used as animal feed. The added value of the product is low and the waste of resources is serious. Further development And making use of this huge potential protein resource, the production of high value-added products has become a top priority

[0005] At present, a large amount of alkali treatment or some harmful chemical solvents are commonly used in the extraction methods of peanut peptides, which inevitably have hidden dangers that threaten human health.

In the existing biological enzyme treatment methods, alkaline protease is generally used to enzymatically hydrolyze peanut protein, and then go through multiple tedious steps such as desalination to prepare peanut peptide. The enzymatic hydrolysis time is generally longer, generally 360-960min The yield and degree of hydrolysis are low, the yield of short peptides is about 60% to 65%, and the degree of hydrolysis is about 12% to 16%. Therefore, how to simplify the process and obtain peanut peptides with high degree of hydrolysis and high yield in a short time become a major problem that needs to be solved in the production process

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