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A method of using microorganism Arthrobacter z3 to treat the transformation of burley tobacco into the flavor type of flue-cured tobacco

A technology of Arthrobacter and Burley tobacco, which is applied in the treatment of tobacco, tobacco, application, etc., and can solve the problems of weakening the characteristic aroma of Burley tobacco, insufficient strength, and insufficient aroma.

Active Publication Date: 2016-03-30
HEILONGJIANG TOBACCO IND
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The main purpose of the present invention is to provide a method for microbial Arthrobacter Z3 to transform Burley tobacco into flue-cured tobacco flavor. The harmonization of flue-cured tobacco smoke can properly solve the shortcomings of "Chinese cigarettes" due to the substantial reduction in the amount of cigarette tar, which leads to insufficient strength, weak smoke taste and insufficient aroma, and has practical significance for improving the quality of cigarette products and brand development

Method used

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  • A method of using microorganism Arthrobacter z3 to treat the transformation of burley tobacco into the flavor type of flue-cured tobacco
  • A method of using microorganism Arthrobacter z3 to treat the transformation of burley tobacco into the flavor type of flue-cured tobacco
  • A method of using microorganism Arthrobacter z3 to treat the transformation of burley tobacco into the flavor type of flue-cured tobacco

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Preparation of Arthrobacter Z3 bacterial solution with an absorbance of 1.2

[0025] K 2 HPO 4 ·3H 2 O1.33%, (NH4) 2 SO 4 0.1%, KH 2 PO 4 0.4%, 0.1% yeast powder, 0.4% nicotine, 0.1% trace elements, adjust the pH to 7, put it into a 250ml Erlenmeyer flask, sterilize at 0.1MPa, 121°C for 30min, and inoculate 5% of the knot at room temperature Bacillus Z3 seed solution was cultivated in a shaker at 30°C and 150 revolutions for about 9 hours. Wherein, the trace elements include 0.02% MgSO 4 ·7H 2 O, 0.04% MnSO 4 ·H 2 O, 0.02% CaCl 2 2H 2 O, dissolved with 0.1mol / L HCl and added water to make up to 100mL, where percentages are mass percentages. At this time, the color of the bacterial solution is light blue, and the ultraviolet spectrum value is 1.2 (the seed solution is used as a control).

[0026] 2. Transformation of burley tobacco into flue-cured tobacco flavor by Arthrobacter Z3 treatment

[0027] Take 100g Hunan Enshi Burley Tobacco B 2 F, take 100m...

Embodiment 2

[0030] Embodiment 2 detects the change of components in Burley tobacco before and after treatment

[0031] 100g Hubei Badong Burley Tobacco C 2 In F2009, 100ml of Arthrobacter Z3 bacterial solution with an absorbance of 1.2 was evenly added to the tobacco leaves by spraying, and fermented dynamically in a fermentation chamber for 72 hours at a temperature of 30°C, a rotational speed of 20 rpm, and a humidity of 50%.

[0032] Use the same steaming method to extract the aroma components, add 350mL distilled water and 60mLCH to both ends of the simultaneous distillation extraction 2 Cl 2 Simultaneously distilled for 2 hours to clean the simultaneous distillation and extraction device to ensure the cleanliness of the instrument. Weigh 30g (accurate to 0.1mg) of smoke powder sample into a 1000mL round bottom flask at one end of the simultaneous distillation extraction device, add appropriate amount of sodium chloride and distilled water, heat with a temperature-controlled electri...

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Abstract

The invention relates to a microbial arthrobacter Z3 treatment method for converting aroma of burley tobaccos into aroma of flue-cured tobaccos. The microbial arthrobacter Z3 treatment method includes uniformly spraying freshly cultured arthrobacter liquid or prepared enzyme liquid on the surfaces of tobacco leaves according to a volume mass proportion of the arthrobacter liquid or the enzyme liquid to the tobacco leaves of 1:0.5-2; allowing the tobacco leaves to ferment in dynamic fermentation tanks for 72h under the conditions of preferable temperature of 30 DEG C, rotational speed of 20 turns / min and humidity of 50%. 100ml of the arthrobacter liquid or the enzyme liquid corresponds to 100g of the tobacco leaves. The microbial arthrobacter Z3 treatment method has the advantages that the characteristic aroma of the burley tobaccos can be obviously weakened, pungent irritation of smoke can be obviously relieved, the aroma of the burley tobaccos is close to the aroma of the flue-cured tobaccos, and the harmony of the aroma of the burley tobaccos and the smoke of the burley tobaccos can be improved; the microbial arthrobacter Z3 treatment method can be used in flue-cured cigarette formulating, so that the concentration of aroma of flue-cured cigarettes can be improved.

Description

technical field [0001] The invention relates to a method for treating Burley tobacco with Arthrobacter Z3 to transform into flue-cured tobacco aroma, in particular to treating Burley tobacco with Arthrobacter Z3 to reduce ammonia and nitrogen-containing basic compounds in Burley tobacco, Therefore, the method for transforming the aroma type of flue-cured tobacco belongs to the technical field of microorganism application. Background technique [0002] In recent years, "Chinese-style cigarettes" have made great progress in reducing harm and coke. However, a significant reduction in the amount of tar in cigarettes will undoubtedly lead to a lighter taste and insufficient aroma, making it difficult for consumers to accept. Therefore, how to keep the unique style, aroma concentration and strength of Chinese cigarettes as much as possible while reducing the tar content of "Chinese cigarettes" has become a new topic for cigarette product developers. Burley tobacco has a loose ti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B15/20
Inventor 安毅马林石中金徐丽霞张相辉张丽李辉帖金鑫关体青魏松江陶冶岳先领周碧波
Owner HEILONGJIANG TOBACCO IND
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