Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method utilizing ion-exchange resin to decolor crude polysaccharide in ganoderma sinensis submerged fermentation mycelium

A technology of liquid submerged fermentation and ion exchange resin, applied in the field of traditional Chinese medicine chemistry

Inactive Publication Date: 2014-06-25
SHANGHAI INST OF PHARMA IND +1
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most literatures at home and abroad adopt the orthogonal test model to optimize the decolorization process of polysaccharides from Ganoderma lucidum, but there are no reports on the optimal decolorization process of polysaccharides fermented from Zizhi lucidum based on response surface methodology (RSM).

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method utilizing ion-exchange resin to decolor crude polysaccharide in ganoderma sinensis submerged fermentation mycelium
  • Method utilizing ion-exchange resin to decolor crude polysaccharide in ganoderma sinensis submerged fermentation mycelium
  • Method utilizing ion-exchange resin to decolor crude polysaccharide in ganoderma sinensis submerged fermentation mycelium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: resin screening

[0045] Take 0.5g of each of the five processed resins, put them into conical flasks with stoppers, add 10ml of refined Zizhi polysaccharide solution to each bottle, place them in a shaker, rotate at 120r / min, keep a constant temperature at 30°C, and shake for 3h. The results are shown in figure 1 .

[0046] The adsorption of macromolecular substances by resin is mainly carried out in two ways: one is the ion exchange between the ions on the resin and the adsorbed substance to adsorb it; The substance is adsorbed. In addition to mainly examining the decolorization rate, the decolorization effect also needs to take into account the index of polysaccharide recovery rate. Depend on figure 1 It can be seen that the decolorization effect of LS-850 anion exchange resin is much better than that of other types of resins (such as figure 1 shown). The anions in LS-850 are exchanged with the pigment anions in Zizhi polysaccharide in the form of ...

Embodiment 2

[0048] Embodiment 2: the impact of decolorization time

[0049] Accurately measure 10ml 5 parts of Zizhi polysaccharide solution, under the same resin dosage LS-8500.5g, temperature 30℃, pH value 6.0, investigate the effect of different time on decolorization, the results are shown in figure 2 .

[0050] Depend on figure 2 It can be seen that with the prolongation of time, the decolorization effect of the resin is obviously enhanced. After 3 hours of LS-850 resin, the decolorization rate begins to increase slowly and has a decreasing trend, indicating that the adsorption and desorption activities of the pigment begin to develop in the direction of dynamic equilibrium. However, the polysaccharide yield basically showed the opposite trend to the decolorization rate, and after 4 hours, the polysaccharide yield almost no longer decreased. In order to remove more pigments in polysaccharides and reduce the loss of polysaccharides as much as possible, considering two indicators c...

Embodiment 3

[0051] Embodiment 3: the influence of resin consumption

[0052] Accurately measure 10ml 5 parts of Zizhi polysaccharide solution, under the same decolorization time of 3h, temperature of 30°C, and pH value of 6.0, investigate the effect of different resin dosage on decolorization, the results are shown in image 3 .

[0053] Depend on image 3 It can be seen that as the amount of resin increases, the decolorization rate shows an upward trend, but when the amount of resin reaches 0.5g, the increasing trend of decolorization rate slows down significantly, and the recovery rate of polysaccharides continues to decline at this time. For subsequent scientific research or further industrial processing, increase the decolorization effect as much as possible while taking into account the higher recovery rate of polysaccharides and reduce the loss rate of polysaccharides, so the resin dosage response surface analysis of the method of the present invention is preferably 0.25g ~ 0.75g ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
decolorization rateaaaaaaaaaa
decolorization rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method utilizing ion-exchange resin to decolor crude polysaccharide in ganoderma sinensis submerged fermentation mycelium. The method comprises the following steps: (a) carrying out a pretreatment on crude polysaccharide in ganoderma sinensis submerged fermentation mycelium so as to obtain refined ganoderma sinensis polysaccharide with a molecular range of 30 KD to 300 KD; (b) transferring the refined ganoderma sinensis polysaccharide obtained in the step (a) to an ion-exchange resin to carrying out a decoloring treatment so as to obtain the discolored ganoderma sinensis polysaccharide, wherein in the decoloring treatment, the conditions are as follows: the decoloring time is 2 to 4 hours, the using amount of resin is 0.25 to 0.75 g / mL, the decoloring temperature is 25 to 35 DEG C, and the pH value is 6 to 7. The actual decoloring rate of the discolored ganoderma sinensis polysaccharide is 85% to 90%, and the recovery rate of polysaccharide is 65% to 70%.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine chemistry, in particular to a method for decolorizing the polysaccharide liquid of Zizhi liquid submerged fermentation mycelia by using an ion exchange resin. Background technique [0002] Ganoderma sinense (Ganoderma sinense) is one of the famous and precious traditional Chinese medicine Ganoderma lucidum (National Pharmacopoeia Committee. The People's Republic of China Pharmacopoeia [S] 2010 Edition Part One Beijing: China Medical Science and Technology Press, 2010: 174), is a treasure in the treasure house of traditional Chinese medicine in the motherland , known as "immortal grass". Ancient and modern pharmacological and clinical studies have proved that Zizhi does have the effect of preventing and curing diseases and prolonging life. The "Shen Nong's Materia Medica" in the Eastern Han Dynasty and the "Compendium of Materia Medica" by Li Shizhen, a famous medical scientist in the M...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 杨义芳胡晓杨悦文罗永明吴春珍屈晓晟黄春跃
Owner SHANGHAI INST OF PHARMA IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com