Heat-resistant freezing and drying protecting agent for contagious ovine ecthyma virus cell attenuated vaccine as well as preparation method and application of heat-resistant freezing and drying protecting agent

A technology of heat-resistant freeze-drying and pustular virus, applied in the field of biomedicine, can solve the problems of vaccine storage and transportation difficulties, inconvenient storage and transportation, complicated manufacturing process, etc., achieve simple formula ingredients and preparation methods, and realize large-scale production , the effect of convenient transportation

Active Publication Date: 2014-07-02
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, among animal vaccines, live freeze-dried vaccines account for a considerable proportion. The protective agents commonly used in China are mainly milk, sucrose, gelatin, etc. Although they have stable shape, good solubility, low cost and convenient storage, they are thermally stable Poor performance and protection, and the product has high requirements on the cold chain
At 2-8°C, the storage period is only 3-6 months, and most of them need to be stored below -15°C, which brings great difficulties to the storage and transportation of vaccines.
The disease is prevalent in many large sheep breeding farms in China, causing seri...

Method used

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  • Heat-resistant freezing and drying protecting agent for contagious ovine ecthyma virus cell attenuated vaccine as well as preparation method and application of heat-resistant freezing and drying protecting agent
  • Heat-resistant freezing and drying protecting agent for contagious ovine ecthyma virus cell attenuated vaccine as well as preparation method and application of heat-resistant freezing and drying protecting agent
  • Heat-resistant freezing and drying protecting agent for contagious ovine ecthyma virus cell attenuated vaccine as well as preparation method and application of heat-resistant freezing and drying protecting agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] [Example 1] Preparation of heat-resistant lyoprotectant (calculated according to the preparation of 1L)

[0024] The preparation method of the heat-resistant freeze-drying protective agent of sheep infectious impetigo virus cell attenuated vaccine comprises the following steps:

[0025] 1) Weigh 50g of trehalose, 20g of skimmed milk, 20g of polyvinylpyrrolidone, and 10g of gelatin, add the above-mentioned substances obtained by weighing into 500mL ultrapure water, heat to 55°C to dissolve, and then sterilize at 116°C for 30 minutes. A liquid;

[0026] 2) Weigh 1.5g of arginine and 2.5g of vitamin C and dissolve them in 200mL of ultrapure water, filter and sterilize with a filter membrane with a pore size of 0.22μm to make liquid B;

[0027] 3) Mix the sterilized liquid A and liquid B, and dilute to 1L, which is the freeze-drying protective agent.

Embodiment 2

[0028] [Example 2] Preparation of heat-resistant lyoprotectant (calculated according to the preparation of 1L)

[0029] The preparation method of the heat-resistant freeze-drying protective agent of sheep infectious impetigo virus cell attenuated vaccine comprises the following steps:

[0030] 1) Weigh 30g of trehalose, 30g of skimmed milk powder, 10g of polyvinylpyrrolidone, and 15g of gelatin, add the above-mentioned substances obtained by weighing into 500mL ultrapure water, heat to 50°C to dissolve, and sterilize at 116°C for 30 minutes to prepare A liquid;

[0031] 2) Weigh 0.5g of arginine and 3.5g of vitamin C, dissolve them in 200mL of ultrapure water, filter and sterilize them with a filter membrane with a pore size of 0.22μm, and make liquid B;

[0032] 3) Mix the sterilized liquid A and liquid B, and dilute to 1L, which is the freeze-drying protective agent.

Embodiment 3

[0033] [Example 3] Preparation of heat-resistant lyoprotectant (calculated according to the preparation of 1L)

[0034] 1) Weigh 70g of trehalose, 10g of skimmed milk powder, 30g of polyvinylpyrrolidone, and 5g of gelatin, add the above-mentioned substances obtained by weighing into 500mL of ultrapure water, heat to 60°C to dissolve, and then sterilize at 116°C for 30 minutes. A liquid;

[0035] 2) Weigh 4g of arginine, dissolve 1g of vitamin C in 200mL of ultra-pure water, filter and sterilize with a filter membrane with a pore size of 0.22 μm, and make liquid B;

[0036] 3) Mix the sterilized liquid A and liquid B, and dilute to 1L, which is the freeze-drying protective agent.

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Abstract

The invention discloses a heat-resistant freezing and drying protecting agent for a contagious ovine ecthyma virus cell attenuated vaccine as well as a preparation method and application of the heat-resistant freezing and drying protecting agent. According to the characteristics of the contagious ovine ecthyma virus cell attenuated vaccine, the heat-resistant freezing and drying protecting agent for the contagious ovine ecthyma virus cell attenuated vaccine is prepared, wherein the heat-resistant freezing and drying protecting agent contains 30g/L-70g/L of trehalose, 10g/L-30g/L of skim milk powder, 10g/L-30g/L of polyvinylpyrrolidone, 5g/L-15g/L of gelatin, 0.5g/L-4g/L of arginine, 1g/L-5g/L of vitamin C, and the balance being of ultra-pure water. By virtue of the heat-resistant freezing and drying protecting agent disclosed by the invention, the vaccine can be stored for 24 months at 2 DEG C-8 DEG C while viral titer is not changed greatly; before and after freezing and drying a vaccine product, virus loss ratio is 0.3 titer, and the virus loss ratio is only 1.0 titer while the vaccine is stored for 30 days at 37 DEG C. According to the heat-resistant freezing and drying protecting agent disclosed by the invention, a bottleneck technology that the contagious ovine ecthyma virus cell attenuated vaccine only can be stored and transported at (-)15 DEG C is solved, so that the vaccine is more convenient to store and transport.

Description

technical field [0001] The invention relates to a heat-resistant freeze-drying protectant for live vaccines and a preparation method thereof, in particular to a heat-resistant freeze-drying protectant for the preparation of an attenuated vaccine of sheep infectious impetigo virus cells and a preparation method thereof. The invention belongs to the technical field of biomedicine. Background technique [0002] Lyoprotectant and its preparation process are a key technology in the production of live vaccines, which have a great impact on the storage, transportation and use of vaccines. At present, among animal vaccines, live freeze-dried vaccines account for a considerable proportion. The protective agents commonly used in China are mainly milk, sucrose, gelatin, etc. Although they have stable shape, good solubility, low cost and convenient storage, they are thermally stable. The safety and protection functions are poor, and the product has high requirements for the cold chain....

Claims

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Application Information

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IPC IPC(8): A61K47/46
Inventor 刘湘涛王光祥尚佑军张志东王艳华黎摄儿田宏吴锦艳陈妍逯忠新
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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