Heat-resistant freezing and drying protecting agent for contagious ovine ecthyma virus cell attenuated vaccine as well as preparation method and application of heat-resistant freezing and drying protecting agent

Abstract

The invention discloses a heat-resistant freezing and drying protecting agent for a contagious ovine ecthyma virus cell attenuated vaccine as well as a preparation method and application of the heat-resistant freezing and drying protecting agent. According to the characteristics of the contagious ovine ecthyma virus cell attenuated vaccine, the heat-resistant freezing and drying protecting agent for the contagious ovine ecthyma virus cell attenuated vaccine is prepared, wherein the heat-resistant freezing and drying protecting agent contains 30g / L-70g / L of trehalose, 10g / L-30g / L of skim milk powder, 10g / L-30g / L of polyvinylpyrrolidone, 5g / L-15g / L of gelatin, 0.5g / L-4g / L of arginine, 1g / L-5g / L of vitamin C, and the balance being of ultra-pure water. By virtue of the heat-resistant freezing and drying protecting agent disclosed by the invention, the vaccine can be stored for 24 months at 2 DEG C-8 DEG C while viral titer is not changed greatly; before and after freezing and drying a vaccine product, virus loss ratio is 0.3 titer, and the virus loss ratio is only 1.0 titer while the vaccine is stored for 30 days at 37 DEG C. According to the heat-resistant freezing and drying protecting agent disclosed by the invention, a bottleneck technology that the contagious ovine ecthyma virus cell attenuated vaccine only can be stored and transported at (-)15 DEG C is solved, so that the vaccine is more convenient to store and transport.

Description

technical field [0001] The invention relates to a heat-resistant freeze-drying protectant for live vaccines and a preparation method thereof, in particular to a heat-resistant freeze-drying protectant for the preparation of an attenuated vaccine of sheep infectious impetigo virus cells and a preparation method thereof. The invention belongs to the technical field of biomedicine. Background technique [0002] Lyoprotectant and its preparation process are a key technology in the production of live vaccines, which have a great impact on the storage, transportation and use of vaccines. At present, among animal vaccines, live freeze-dried vaccines account for a considerable proportion. The protective agents commonly used in China are mainly milk, sucrose, gelatin, etc. Although they have stable shape, good solubility, low cost and convenient storage, they are thermally stable. The safety and protection functions are poor, and the product has high requirements for the cold chain....

AI Technical Summary

Problems solved by technology

At present, among animal vaccines, live freeze-dried vaccines account for a considerable proportion. The protective agents commonly used in China are mainly milk, sucrose, gelatin, etc. Although they have stable shape, good solubility, low cost and convenient storage, they are thermally stable Poor performance and protection, and the product has high requirements on the cold chain

At 2-8°C, the storage period is only 3-6 months, and most of them need to be stored below -15°C, which brings great difficulties to the storage and transportation of vaccines.

The disease is prevalent in many large sheep breeding farms in China, causing serious economic losses to the sheep industry

Although the attenuated oral ulcer vaccine has been developed in Qinghai Provincial Academy of Animal Husbandry and Veterinary Sciences and Gansu Provincial Animal Husbandry and Veterinary Research Institute, and has applied for local new veterinary drug certificates, but due to the complicated and backward manufacturing process, the formula of the vaccine freeze-dried protective agent is simple , so that the prepared vaccine has poor protection against antigens, and has disadvantages such as inconvenient storage and transportation

At present, it is difficult to buy a mouth sore vaccine on the market

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