Circulating tumor cell in-vivo capturing device and circulating tumor cell in-vivo capturing method on basis of indwelling needles

A tumor cell and capture device technology, applied in the field of circulating tumor cell in vivo capture device, can solve the problems of unrepresentation and false negative, and achieve the effects of low cost, simple operation and high cost efficiency

Inactive Publication Date: 2014-07-09
SHANDONG NORMAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

This will inevitably lead to a non-representative sample taken randomly from the blood, resulting in a high probability of false negatives for such sampling-based ass

Method used

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  • Circulating tumor cell in-vivo capturing device and circulating tumor cell in-vivo capturing method on basis of indwelling needles

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Embodiment 1

[0018] An indwelling needle-based in vivo capture device for circulating tumor cells, including an indwelling needle, the outer surface of the indwelling needle is covered with PDMS, and epithelial cell-specific antibodies for specifically recognizing and capturing circulating tumor cells (target circulating tumor cells) are adsorbed on the PDMS The cells are MCF-7 cells, and the epithelial cell-specific antibody selected is anti-EpCAM antibody, purchased from Beijing Boaosen Biotechnology Co., Ltd.).

[0019] The specifications and models of the indwelling needle are 24G×0.75", 0.7mm×19mm.

[0020] The preparation method of the indwelling needle-based in vivo capture device for circulating tumor cells is as follows:

[0021] (1) PDMS coating: mix the PDMS precursor liquid and its curing agent at a volume ratio of 5:1 to obtain a mixed solution, then immerse the indwelling needle in the mixed solution, rotate the indwelling needle clockwise to make the coating even, and then ...

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Abstract

The invention discloses a circulating tumor cell in-vivo capturing device on the basis of indwelling needles. The circulating tumor cell in-vivo capturing device comprises the indwelling needles. Materials capable of adsorbing proteins wrap the outer surfaces of the indwelling needles, and epithelial cell specific antibodies which are used for recognizing specificity and capturing circulating tumor cells are adsorbed on the materials capable of adsorbing the proteins. The invention further discloses a method for capturing the circulating tumor cells by the aid of the capturing device. The circulating tumor cell in-vivo capturing device and the method have the advantages that patients are transfused through the indwelling needles, so that the circulating tumor cells in the blood vessels of the patients can be captured by the modified specific antibodies on the indwelling needles during transfusion; the indwelling needles are pulled out of the patients after the transfusion is completed, so that the captured circulating tumor cells can be removed from the bodies of the patients, and are eluted by eluent; the eluted cells can be observed and counted under microscopes, or immunostaining can be carried out on target molecules to be tested in the cells, and the target molecules to be tested in the cells can be observed through confocal microscopes; the circulating tumor cell in-vivo capturing method is simple in operation, and the target cells can be efficiently and selectively captured by the aid of the circulating tumor cell in-vivo capturing method.

Description

technical field [0001] The invention relates to a device and method for capturing circulating tumor cells in vivo based on an indwelling needle. Background technique [0002] It has been recognized for decades that cancer metastasis is largely due to the dissemination of cancer cells into the blood by carcinoma in situ, where they are carried to other organs to form new tumors. Currently, the capture and detection of circulating tumor cells (CTCs) has been used as a new liquid diagnostic method to track the spread of cancer or predict the prognosis of treatment. So far, almost all methods for capturing and detecting CTCs are based on taking several milliliters of samples from blood, and then capturing and detecting CTCs in this sample. The basic rule of sampling in analytical chemistry is that the sample should be representative, but the dissemination of CTCs is irregular, and occasionally a group of cells will disseminate into the blood, so the distribution of CTCs in the ...

Claims

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Application Information

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IPC IPC(8): A61B10/02A61M5/158
Inventor 张鸿雁贾真珍武传琛唐波
Owner SHANDONG NORMAL UNIV
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