Circulating tumor cell in-vivo capturing device and circulating tumor cell in-vivo capturing method on basis of indwelling needles

Abstract

The invention discloses a circulating tumor cell in-vivo capturing device on the basis of indwelling needles. The circulating tumor cell in-vivo capturing device comprises the indwelling needles. Materials capable of adsorbing proteins wrap the outer surfaces of the indwelling needles, and epithelial cell specific antibodies which are used for recognizing specificity and capturing circulating tumor cells are adsorbed on the materials capable of adsorbing the proteins. The invention further discloses a method for capturing the circulating tumor cells by the aid of the capturing device. The circulating tumor cell in-vivo capturing device and the method have the advantages that patients are transfused through the indwelling needles, so that the circulating tumor cells in the blood vessels of the patients can be captured by the modified specific antibodies on the indwelling needles during transfusion; the indwelling needles are pulled out of the patients after the transfusion is completed, so that the captured circulating tumor cells can be removed from the bodies of the patients, and are eluted by eluent; the eluted cells can be observed and counted under microscopes, or immunostaining can be carried out on target molecules to be tested in the cells, and the target molecules to be tested in the cells can be observed through confocal microscopes; the circulating tumor cell in-vivo capturing method is simple in operation, and the target cells can be efficiently and selectively captured by the aid of the circulating tumor cell in-vivo capturing method.

Description

technical field [0001] The invention relates to a device and method for capturing circulating tumor cells in vivo based on an indwelling needle. Background technique [0002] It has been recognized for decades that cancer metastasis is largely due to the dissemination of cancer cells into the blood by carcinoma in situ, where they are carried to other organs to form new tumors. Currently, the capture and detection of circulating tumor cells (CTCs) has been used as a new liquid diagnostic method to track the spread of cancer or predict the prognosis of treatment. So far, almost all methods for capturing and detecting CTCs are based on taking several milliliters of samples from blood, and then capturing and detecting CTCs in this sample. The basic rule of sampling in analytical chemistry is that the sample should be representative, but the dissemination of CTCs is irregular, and occasionally a group of cells will disseminate into the blood, so the distribution of CTCs in the ...

AI Technical Summary

Problems solved by technology

This will inevitably lead to a non-representative sample taken randomly from the blood, resulting in a high probability of false negatives for such sampling-based assays

Even if the established assay can detect 1 cell in 1 mL of blood, this is not an accurate result relative to the uneven distribution of CTCs in the whole body blood

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