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Method for rapid identification of microalgae on single cell level

A single-cell, microalgae technology, used in material excitation analysis, Raman scattering, etc., to avoid errors and reduce sample requirements

Inactive Publication Date: 2014-07-23
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Claims
  • Application Information

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Problems solved by technology

The application of single-cell Raman spectroscopy to identify microalgae has not been reported

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  • Method for rapid identification of microalgae on single cell level
  • Method for rapid identification of microalgae on single cell level
  • Method for rapid identification of microalgae on single cell level

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Embodiment

[0024] 1) Four strains of Chlamydonomas reinhardtii mutants CC124, CC4324, CC4333 and CC4334 were purchased from Chlamydomonas Resource Center: http: / / chlamycollection.org / strains / .

[0025] 2) Chlamydomonas reinhardtii was inoculated into TAP (Tris acetate phosphate) liquid medium with or without nitrogen (3 biological replicates), 150 μmol photons m -2 the s -1 Light, 25 ° C, aeration and stirring culture. Samples were taken when culturing to 0h, 12h, 24h, 48h, 72h and 96h. Among them, TAP liquid medium with nitrogen or nitrogen deficiency, TAP (Tris acetate phosphate) medium:

[0026] NH 4 Cl (7.48mM), MgSO 4 (406 μM), CaCl 2 (340μM), K 2 HPO 4 (540μM), KH 2 PO 4 (463μM), 20mM Tris, 17.4mM acetate, H 3 BO 3 (184μM), ZnSO 4 (76.5μM),MnCl 2 (25.5μM), FeSO 4 (17.9 μM), CoCl 2 (6.77μM),(NH4) 6 Mo 7 o 24 (0.88μM), CuSO 4 (6.29μM), and Na 2 EDTA (148μM)

[0027] 3) Take the algae liquid of different culture time in step 2), take 1mL of the algae liquid for ea...

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Abstract

The invention relates to the field of microalgae identification, and in particular relates to a method for rapid identification of microalgae on a single cell level based on a single cell Raman spectrum. The method comprises the following steps: collecting single cell Raman spectrums of microalgae cells, establishing a microalgae single cell Raman spectrum database, and comparing a single cell Raman spectrum of unknown microalgae with the established microalgae single cell Raman spectrum database, thus realizing microalgae identification or mutant screening on the single cell level. The method disclosed by the invention identifies the microalgae through a single cell Raman spectrum technology; a simple, rapid and non-invasive microalgae identification method on the single cell level is provided, thus providing an efficient discrimination method for microalgae identification and mutant screening.

Description

technical field [0001] The invention relates to the field of microalgae identification, in particular to a method for rapidly identifying microalgae at the single-cell level based on single-cell Raman spectroscopy. Background technique [0002] Microalgae are a type of photoautotrophic microorganisms that can utilize light energy and CO 2 Synthesize proteins, sugars, lipids, pigments and other substances and release O2; microalgae are the primary producers in the ecosystem with many types, large numbers, and rapid reproduction; they are also an important part of the material and energy cycle of the ecosystem; Algae have broad application prospects in the fields of food, health care, medicine, environmental protection and new biofuel refining. For example, Chlorella, Scenedesmus, Crescentella, Spirulina, etc. have high protein content and have been used as protein sources; Chlorella, Spirulina, Dunaliella salina, Haematococcus pluvialis, etc. are rich in unsaturated Fatty a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 徐健何曰辉籍月彤王婷婷王允黄巍
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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