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Immunofluorescence test strip for fast and quantitatively detecting curative effect of aspirin and preparation method of immunofluorescence test strip

A quantitative detection and aspirin technology, applied in the field of clinical medical diagnosis, can solve the problems of high cost, long detection time, unsuitable for clinical rapid diagnosis, etc., and achieve the effect of accurate results and simple operation

Inactive Publication Date: 2014-07-23
RELIA BIOTECH JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the method for detecting 11-dehydrothromboxane (11dhTxB2) is mainly enzyme-linked immunoassay (ELISA). ELISA technology has the following disadvantages: high requirements for detection equipment and high cost; many interference factors and poor reproducibility; detection long time
Therefore, ELISA detection of 11dhTxB2 is not suitable for rapid clinical diagnosis

Method used

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  • Immunofluorescence test strip for fast and quantitatively detecting curative effect of aspirin and preparation method of immunofluorescence test strip
  • Immunofluorescence test strip for fast and quantitatively detecting curative effect of aspirin and preparation method of immunofluorescence test strip
  • Immunofluorescence test strip for fast and quantitatively detecting curative effect of aspirin and preparation method of immunofluorescence test strip

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Embodiment 1

[0039] Preparation of immunofluorescence test strips for rapid quantitative detection of aspirin efficacy

[0040] Such as figure 1 The immunofluorescence test strip structure shown is an immunofluorescence test strip for rapid quantitative detection of aspirin drug efficacy, which includes a test strip support sheet 4 and sample pads 1, Detection membrane 2 and water-absorbing gasket 3, a coupling gasket 5 is arranged between the sample gasket 1 and the detection membrane 2, and a first layer of glass fiber gasket 6-1 is arranged above one end of the coupling gasket 5. A second layer of glass fiber gasket 6-2 is provided below one end or only a first layer of glass fiber gasket 6-1 is provided above one end of the coupling gasket 5 or no gasket is provided. A detection line 7 is provided, and the detection line 7 is coated with 11-dehydrothrombin B2 (11dhTxB2) monoclonal antibody or polyclonal antibody, and the other side of the detection line 7 is provided with a control li...

Embodiment 2

[0057] Quick and quantitative detection of aspirin curative effect with immunofluorescence test strips of the present invention

[0058] Get the immunofluorescence test strip of a kind of fast quantitative detection aspirin curative effect preferably of the present invention, as figure 1 The immunofluorescence test strip structure shown is an immunofluorescence test strip for rapid quantitative detection of aspirin drug efficacy, which includes a test strip support sheet 4 and sample pads 1, Detection membrane 2 and water-absorbing gasket 3, a coupling gasket 5 is arranged between the sample gasket 1 and the detection membrane 2, and a first layer of glass fiber gasket 6-1 is arranged above one end of the coupling gasket 5. A second layer of glass fiber gasket 6-2 is provided below one end or only a first layer of glass fiber gasket 6-1 is provided above one end of the coupling gasket 5 or no gasket is provided. A detection line 7 is provided, and the detection line 7 is coat...

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Abstract

The invention discloses an immunofluorescence test strip for fast and quantitatively detecting the curative effect of aspirin and a preparation method of the immunofluorescence test strip. The immunofluorescence test strip comprises a support piece as well as a sample gasket, a detection film and a water absorption gasket, which are sequentially overlapped and pasted on the support piece, wherein a conjugate gasket is arranged between the sample gasket and the detection film; a first layer of glass fiber gasket is arranged above one end of the conjugate gasket and a second layer of glass fiber gasket is arranged below one end of the conjugate gasket or only the first layer of glass fiber gasket is arranged above one end of the conjugate gasket or no gasket is arranged; a detection line is arranged on the detection film; the detection line is coated with a 11-dehydro thromboxane B2 (11dhTxB2) monoclonal antibody or polyclonal antibody; a control line is arranged on the other side of the detection line; the control line is coated with an anti-streptavidin (SAV) antibody; the conjugate gasket is coated with fluorescently labeled 11dhTxB2 conjugate. The immunofluorescence test strip is convenient, efficient, simple to operate, accurate in result and suitable for rapid clinical diagnosis.

Description

technical field [0001] The invention belongs to the field of clinical medical diagnosis, and in particular relates to an immunofluorescence test strip for quantitative detection of aspirin and a preparation method thereof. Background technique [0002] Aspirin is widely used as an auxiliary prescription to prevent cardiovascular diseases because it can inhibit the generation of thromboxane A2 (TxA2), which can effectively constrict blood vessels and promote platelet aggregation. Although aspirin is consumed by millions of people worldwide each year, recent studies have shown that not all individuals respond to aspirin in the same way. Studies have shown that cholesterol, triglycerides, and C-reactive protein levels are reduced in response to aspirin in coronary heart disease and related patients using blood-based platelet aggregation and urine 11dhTxB2 assays. Compared with asymptomatic patients, patients with coronary artery disease showed significantly higher urinary 11dh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/558G01N33/54386G01N33/9486G01N2500/20
Inventor 刘红剑威廉姆·努特何小红刘丽萍张丹
Owner RELIA BIOTECH JIANGSU
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