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Preparation method of squid active peptide

A technology of active peptide and squid, which is applied in the field of preparation of active peptide, can solve the problems that restrict the deep processing and application of squid, and achieve the effect of short time, high efficiency and less by-products

Active Publication Date: 2014-07-30
BEIJING SEMNL BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are deficiencies in the existing methods, which restrict the deep processing and application of squid to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting squid active peptide from squid, comprising the steps of:

[0023] a. Raw material processing: wash 10kg of fresh squid and add 10kg of pure water to mince;

[0024] b. Decolorization and ultrasonic treatment: Add 4kg of 0.01mol / L KOH solution to the minced squid, ultrasonically treat it for 20min, the action temperature is 65°C, the power is 350W, wash with pure water until the pH is neutral, filter to obtain squid protein extract;

[0025] c. High pressure treatment: put the squid protein extract obtained in step b into a high pressure tank and pressurize to 3kgf / cm 2 , and keep it for 10 minutes to make the pressure uniform, and then suddenly reduce the pressure to Okgf / cm in 0-3 seconds 2 , filtered to obtain squid active peptide 1.52kg, content 91.5%.

Embodiment 2

[0027] A method for extracting squid active peptide from squid, comprising the steps of:

[0028] a. Raw material processing: wash 10kg of fresh squid and add 40kg of pure water to mince;

[0029] b. Decolorization and ultrasonic treatment: Add 2 kg of 0.50 mol / L NaOH solution to the minced squid, ultrasonically treat it for 40 minutes, operate at a temperature of 50°C, and use a power of 300W, wash with pure water until the pH is neutral, and filter to obtain squid protein extract;

[0030] c. High pressure treatment: put the squid protein extract obtained in step b into a high pressure tank and pressurize to 3kgf / cm 2 , and keep it for 20 minutes to make the pressure uniform, and then suddenly reduce the pressure to Okgf / cm in 0-3 seconds 2 , filtered to obtain squid active peptide 1.48kg, content 92.1%.

Embodiment 3

[0032] A method for extracting squid active peptide from squid, comprising the steps of:

[0033] a. Raw material processing: wash 10kg of fresh squid and add 20kg of pure water to mince;

[0034] b. Decolorization and ultrasonic treatment: Add 3kg of 0.25mol / L NaOH solution to the minced squid, ultrasonic treatment for 30min, the action temperature is 65°C, the power is 300W, wash with pure water until the pH is neutral, filter to obtain squid protein extract;

[0035] c. High pressure treatment: put the squid protein extract obtained in step b into a high pressure tank and pressurize to 3kgf / cm 2 , and keep it for 15 minutes to make the pressure uniform, and then suddenly reduce the pressure to Okgf / cm in 0-3 seconds 2 , filtered to obtain squid active peptide 1.55kg, content 93.4%.

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PUM

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Abstract

The invention discloses a method for extracting squid active peptide from squids. The method comprises the following steps: raw material treatment, ultrasonic treatment and high-pressure treatment to obtain a finished product. Compared to the prior art, the preparation method greatly shortens time.

Description

technical field [0001] The invention relates to a preparation method of active peptide, in particular to a method for extracting squid active peptide from squid. Background technique [0002] Squid, also known as squid and squid, belongs to molluscs and is a kind of squid. Squid is a rare seafood and an important marine cephalopod aquatic product, widely distributed in the Atlantic Ocean, Indian Ocean and Pacific Ocean. The annual catch of squid in my country is about 500,000 tons, which is the main species of pelagic fishing in China. It is also one of the species with the most development potential in pelagic fishery. Squid is rich in calcium, phosphorus, and iron, which is good for bone development and hematopoiesis, and can effectively treat anemia; squid also contains a lot of taurine, which can inhibit the cholesterol content in the blood, relieve fatigue, restore vision, and improve liver function; Containing peptides and selenium have anti-viral and anti-ray effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04
Inventor 归三岷
Owner BEIJING SEMNL BIOTECHNOLOGY CO LTD
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