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A kind of method utilizing burdock leaf to prepare mold inhibitor

A technology of burdock leaf and inhibitor, applied in the field of natural products, can solve the problem of less mold inhibitor and the like, and achieve the effects of improving the extraction rate and shortening the extraction time.

Active Publication Date: 2016-12-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on fungal inhibitors in burdock leaves, and there is no report on the preparation of fungal inhibitors from burdock leaves by ultrasonic microwave-assisted extraction and column chromatography

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take 5 g of dried burdock leaves, crush the dried burdock leaves to below 100 mesh, then mix the burdock leaf powder with water, and the mass ratio of the burdock leaf powder to water is 1:10. Add compound enzyme and carry out enzymolysis, the mass ratio of cellulase and protease is 1: 1, the addition amount of compound enzyme is 1mg / g (burdock leaf), filter after enzymolysis is finished, obtain the burdock leaf aqueous extract, 50 ℃ reduce Concentrate under reduced pressure until there is no solvent to obtain the concentrate a. The obtained burdock leaf residue is set aside.

[0014] Add 10% ethanol solution to the burdock leaf residue as a solvent, the mass ratio of the ethanol solution to the burdock leaf powder used at the beginning of the experiment is 2: 1, and carry out ultrasonic and microwave cooperative extraction. The ultrasonic power is 500w, and the microwave power is 200w. The time is 1min, filter. The supernatant was concentrated under reduced pressure ...

Embodiment 2

[0016] Grinding the dried burdock leaves to below 50 mesh, mixing 100g of burdock leaf powder with water, the mass ratio of burdock leaf powder to water is 1:5. Add compound enzyme to carry out enzymolysis, the mass ratio of cellulase and protease is 1: 2, the addition amount of compound enzyme is 0.5mg / g (burdock leaf), filter after enzymolysis is completed, obtain burdock leaf aqueous extract, 60 ℃ Concentrate under reduced pressure until there is no solvent to obtain the concentrate a. Add 20% ethanol solution to the burdock leaf residue as a solvent, the mass ratio of the ethanol solution to the burdock leaf powder used at the beginning of the experiment is 10:1, and carry out ultrasonic and microwave cooperative extraction. The ultrasonic power is 1Kw, and the microwave power is 1Kw. The time is 8min, filter. The supernatant was concentrated under reduced pressure at 55° C. to no solvent to obtain concentrate b. Combine the concentrates a and b and disperse them in wate...

Embodiment 3

[0018] Take 1000 g of burdock leaves, crush them to below 200 mesh, mix the burdock leaf powder with 40 liters of water, add 20 g of compound enzymes for enzymolysis, the mass ratio of cellulase and protease is 2:1, filter after the enzymolysis is completed, and obtain burdock leaves The aqueous extract was concentrated under reduced pressure at 65°C until there was no solvent to obtain the concentrate a. Add 4000ml of 35% ethanol solution to the burdock leaf residue as a solvent for ultrasonic and microwave co-extraction, the ultrasonic power is 1Kw, the microwave power is 2Kw, the extraction time is 20min, and filtered. The supernatant was concentrated under reduced pressure at 60° C. to no solvent to obtain concentrate b. Combine the concentrates a and b and disperse them in water. The ratio of the combined concentrate to water is 1:9 to obtain a suspension, which is subjected to chromatography on a macroporous resin column. The mass ratio of the suspension to the macroporo...

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Abstract

The invention relates to a method for preparation of a mould inhibitor from burdock leaves. The method is characterized by comprising the steps of: fully crushing dry burdock leaves, adding water to conduct enzymolysis by a composite enzyme to obtain a burdock leaf water extract, and conducting concentration to obtain a concentrate a; then adding an ethanol solution into the burdock leaf residue left over by enzymolysis, carrying out ultrasound-microwave synergistic extraction, filtration and concentration to obtain an extract b; combining the concentrates a and b, dispersing the mixture in water, subjecting the obtained solution to chromatography on a macroporous resin column, and conducting eluting by the solvent, thus obtaining an ethanol eluate, i.e. the mould natural inhibitor. The method provided by the invention utilizes the ultrasound-microwave synergistic extraction technology and the bio-enzyme technology to conduct extraction, greatly shortens the extraction time, and improves the extraction rate. Then the column chromatography technology is employed to conduct purification, thus obtaining the mould natural inhibitor with strong activity.

Description

technical field [0001] The invention belongs to the field of natural products, and relates to a method for preparing a natural mold inhibitor by using burdock leaves as a raw material, in particular a method for preparing a natural mold inhibitor by using ultrasonic and microwave cooperative extraction technology, enzyme technology and column chromatography technology. Background technique [0002] According to statistics, my country's burdock output is large, such as the yield per mu of burdock root in Xuzhou area reaches 1500Kg, and the leaves are about 1000Kg per mu. The burdock leaves are basically all discarded, causing a serious waste of resources and easily polluting the environment. The active ingredients contained in burdock leaves have antibacterial ability. Therefore, the development and utilization of polyphenolic components in burdock leaves can not only comprehensively utilize waste resources, but also prepare a large number of mold-inhibiting active component...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/12A01P3/00
Inventor 娄在祥王洪新李雅芹
Owner JIANGNAN UNIV
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