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Synthetic root-specific promoter and application thereof

An artificial synthesis and promoter technology, applied in the biological field, can solve problems such as root rot infection, susceptibility to underground pests, and increase in bacterial and fungal diseases

Active Publication Date: 2014-08-06
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant roots are easily eaten by underground pests, and the probability of being infected with bacterial and fungal diseases such as root rot and stem rot increases, resulting in reduced yields in some crop production areas

Method used

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  • Synthetic root-specific promoter and application thereof
  • Synthetic root-specific promoter and application thereof
  • Synthetic root-specific promoter and application thereof

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Embodiment Construction

[0022] The present invention is described in further detail below in conjunction with embodiment.

[0023] The experimental materials involved in the following are all published or commercially available materials.

[0024] 1. Experimental steps and methods

[0025] 1.1 Construction of artificial promoters SRS1 and SRS2

[0026] Two promoter sequences, SRS1 and SRS2, were artificially synthesized, and the sequences were chemically synthesized by Jinweizhi Biotechnology (Suzhou) Co., Ltd.

[0027] SEQ ID NO1: SRS1:

[0028] aagcttGATTATTTACTCTTGTAAATAAAAGAGAACGTGATTTACTCTTGTAAATAAAAGAGAACGTGACTGTGTAGCACTGTGTACATAACTGTGTACATTTGAAACTGTGTAAAGTTTTATCTTTAGTTTTTAATCTTTACTTTTAATCTTTTAATAAAGATTAGAGCCTTGAACGGCAAGTTTCACGCTGTCACTTGATAATTAATTCATATTTTAAAAATATTAAATAAATTAATTTTTAATATTTACCAGAAATGATAATTAATTCATATTTTTTTATCAAGCATGCTTCTTGC

[0029] SEQ ID NO2: SRS2:

[0030] aagcttCCATTAGATCTCTTCAATTTCAAAGAGAATCATCCAAACTCTTCAATTTCAAAGAGATTCCTACTGTGTAAGACTGTGTATCTAACTGTGTAGAAAGCAAACTGTGTATTGTAATA...

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Abstract

The invention relates to a synthetic root-specific promoter and application thereof, belonging to the technical field of biology. The synthetic root-specific promoter is named SRS1, and the nucleotide sequence is disclosed as SEQ ID NO 1; 35S promoter partial region and Omega sequence are added by an overlapping PCR process, and the synthetic sequence is SRSO1; by using pCAMB2300.1 as the basic vector (containing GUSplus report gene), the CaMV35S promoter on the pCAMB2300.1 is substituted by the synthetic promoter to obtain the expression vector p2300.1-SRSO1; and the promoter is transformed into agrobacterium, the verification on the promoter transferred into tobacco indicates that the promoter SRS1 has the root-specific expression characteristic. The synthetic promoter SRS1 enriches the varieties of the synthetic root-specific promoters, and can be used for root expression of plant anti-insect genes and for preventing and treating soil insects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a root-specific expression promoter, its cloning, functional verification and its application. Background technique [0002] Tissue-specific promoters can avoid the waste of protein synthesis, so that genes can only be expressed in certain organs or tissues. Tissue-specific promoters discovered successively mainly include root-specific, fruit-specific, vascular tissue-specific, pollen tube-specific, phloem-specific, seed-specific and tuber-specific promoters, which are important tools for studying gene function and expression characteristics. , especially play an important role in plant genetic engineering breeding. [0003] The root is an important organ of the plant. In addition to absorbing water and nutrients in the soil and storing synthetic organic matter, the root also has a close interaction with the microorganisms in the soil. The root system of plants is easily eaten by u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00
Inventor 张杰耿丽丽迟婧束长龙宋福平彭琦梁影屏
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI