Vascular endothelial growth factor quantitative determination kit and light-emitting substrate solution thereof

Abstract

The invention discloses a vascular endothelial growth factor quantitative determination kit and a light-emitting substrate solution thereof. The light-emitting substrate solution comprises a light emitting solution A and a light emitting solution B, wherein the light emitting solution A comprises the following ingredients with concentrations: 0.40-0.50 g / L luminol, 0.10-0.15 g / L p-iodophenol, 0.10-0.15 g / L 1,2-cyclohexanediamine tetraacetic acid, 4.60-5.40 g / L boric acid, 11.00-12.00 g / L borax, and 10.00-12.00 g / L sodium chloride; the light emitting solution B comprises the following ingredients with concentrations: 0.20-0.25 g / L urea peroxide, 1.00-1.50 ml / L Tween 20, 4.60-5.40 g / L boric acid, 11.00-12.00 g / L borax, and 10.00-12.00 g / L sodium chloride; a volume ratio of the light emitting solution A to the light emitting solution B during use is 1:1. The light-emitting substrate solution disclosed by the invention has the advantages of good stability, high sensitivity, and long photon strength platform period.

Description

technical field [0001] The invention relates to a protein immunodetection method, in particular to a kit for quantitative determination of vascular endothelial growth factor and a luminescent substrate solution thereof. Background technique [0002] Serum tumor markers (tpgmor markers, TM) refer to a class of substances produced by tumor cells themselves or by the body's response to tumor cells during the occurrence and proliferation of tumors, reflecting the existence and growth of tumors. Including proteins, hormones, enzymes (isoenzymes), polyamines and oncogene products. The detection of tumor markers is simple and less invasive, so it is widely used in tumor screening, diagnosis, and treatment. [0003] The growth and metastasis of cancer cells depend on the formation of new blood vessels, and vascular endothelial growth factor (VEGF) is the most effective factor for promoting angiogenesis (Ferrara et al. Endocr. Rev. 1997, 18: 4-25). Vascular endothelial growth facto...

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Problems solved by technology

[0006] However, the application of chemiluminescent method is greatly limited due to the disadvantages of poor stability of the luminescent substrate solution used, photon intensity plateau period, and high signal-to-noise ratio.

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