Apparatus and method for screening cell characteristic by bioreactor

A bioreactor and cell technology, applied in the field of bioengineering, can solve the problems of cumbersome operation, long time-consuming, cumbersome procedures, etc., and achieve the effect of reducing the pollution rate, shortening the screening time, and reducing the workload

Active Publication Date: 2014-08-13
上海泰因生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in this process, the operation is cumbersome, and it is easy to cause false positives, cumbersome procedures, high pollution rate, long time-consuming, and heavy workload

Method used

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  • Apparatus and method for screening cell characteristic by bioreactor
  • Apparatus and method for screening cell characteristic by bioreactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0046] Instruments such as bioreactors and reagents such as CHO cells used in this example are commercially available.

[0047] In this embodiment, cell characteristic screening is carried out by the following methods:

[0048] 1) Install pH electrodes, dissolved oxygen electrodes, dissolved carbon dioxide electrodes, liquid level electrodes and temperature electrodes on the bioreactor, and connect these electrodes to a computer equipped with probe group data signal interface and self-made online monitoring system software (ie On the cell culture control device 11), the set temperature is 37°C, the pH value is 7.1, the dissolved oxygen concentration is 45%, and the dissolved carbon dioxide concentration is 5%. The temperature is controlled by the heating jacket, and the electronically adjustable gas flow valve controls the CO 2 , N 2 , O 2 Four-way intake with air, controlled dissolved oxygen concentration and dissolved carbon dioxide concentration by bubbling supply method,...

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Abstract

The invention discloses an apparatus and a method for screening cell characteristic by a bioreactor, the cell characteristic screening method is characterized in that according to different metabolism rate of the cells under different cell states, by adding physical, chemical and biological factors in a cell characteristic screening process to cause the different specific growth rate of the cell, the cell with fast specific growth rate is the screened cell. The apparatus and method can realize the automated management during the screening process and a culture process, and enable continuous characteristic screening to pair cell (such as G418pressurization or continuous serum reduction), a lot of target protein or target cell can be simultaneously obtained while screening, in addition, the screening process of a reactor is capable of greatly shortening the screening time, reducing the workload and reducing the pollution rate, artificial factor can be better removed, and the apparatus can provide the guaranteed cell strain for industrial application.

Description

technical field [0001] The invention relates to a device and method in the field of bioengineering, in particular to a device and method for screening cell characteristics with a bioreactor. Background technique [0002] At present, most of the characteristic cell lines used in bioengineering (such as serum-free, monoclonal stable cell lines) are screened in the traditional way, using well plates or spinner bottles according to the characteristics they need to screen (such as monoclonal stable cell lines) , serum-free cell line) can also meet its basic needs, but its disadvantages are that it is easy to cause false positives, complicated procedures, high pollution rate, long time-consuming, heavy workload, and large human factors. In addition, the in vitro growth environment of cells provided by the traditional method is not easy to control, and usually changes with the culture process, and it is difficult to achieve continuity in the traditional method. [0003] After sear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36C12M1/04C12N5/00
CPCC12M29/00C12M33/00C12M35/00C12M35/08C12M41/48
Inventor 齐念民郑琛傅强庄超
Owner 上海泰因生物技术有限公司
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