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A method for preparing a sample for gas chromatographic analysis of tobacco components and a method for gas chromatographic analysis of tobacco components

A gas chromatographic analysis and gas chromatographic technology, applied in the field of tobacco component analysis, can solve the problems of loss of volatile aroma components, decrease in accuracy, and influence on tobacco leaf components, etc., and achieve the effect of improving detection sensitivity and separation, and avoiding loss

Active Publication Date: 2015-12-30
JILIN TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of the tobacco leaf sample matrix, especially the presence of high peak nicotine, it will affect the gas chromatography behavior of tobacco leaf components, and the above sample pretreatment methods not only have great defects in solvent, sample amount and extraction time, but also During the treatment process, it is difficult to guarantee the original aroma characteristics of tobacco leaves, and it is easy to cause the loss of some volatile aroma components. It is unavoidable to avoid problems such as limited chromatographic peak capacity and insufficient resolution due to nicotine, which makes the analysis of aroma components, pesticide residues and Reduced accuracy of test results for other hazardous substances

Method used

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  • A method for preparing a sample for gas chromatographic analysis of tobacco components and a method for gas chromatographic analysis of tobacco components
  • A method for preparing a sample for gas chromatographic analysis of tobacco components and a method for gas chromatographic analysis of tobacco components
  • A method for preparing a sample for gas chromatographic analysis of tobacco components and a method for gas chromatographic analysis of tobacco components

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preparation example Construction

[0046] The invention provides a method for preparing a tobacco component gas chromatographic analysis sample, comprising the following steps:

[0047] The tobacco sample to be tested is subjected to air-purging micro-extraction to obtain the extract phase. The temperature of the air-purging micro-extraction is 240°C to 260°C, the time of the air-purging micro-extraction is 3min to 5min, and the airflow is The extraction solvent of scanning micro-extraction is n-hexane;

[0048] The extraction phase is mixed with hydrochloric acid solution, and liquid-liquid extraction is carried out to obtain an organic phase;

[0049] The water in the organic phase is removed to obtain a gas chromatography analysis sample of tobacco components.

[0050] The method provided by the present invention adopts n-hexane as the extraction solvent, and carries out air purging micro-extraction on the tobacco sample to be tested at 240°C to 260°C for 3min to 5min; then mixes the obtained extraction pha...

Embodiment 1

[0083] The tobacco leaf sample that this embodiment detects is the middle leaf of flue-cured tobacco, and grade is B 2 F, tobacco leaves before processing and after conditioning;

[0084] Freeze-dry the tobacco leaves and pulverize them with a pulverizer. After passing through a 60-mesh sieve, put the fraction under the sieve into a brown sample bottle and store it at -4°C for future use.

[0085] Accurately weigh 5 tobacco leaf samples of 8 mg each, put them into sample tubes, put the sample tubes into the sample pool, seal the mouth of the sample pool with a septum, place the 200 μL inner tube on the support frame of the condenser, and use The PTFE tube is connected with two needles, one end is put into the 200μL inner tube until it just reaches the bottom of the liner, the other end is inserted into the sample cell along the sealing gasket until it just passes through the lower part of the sealing gasket, and the 200μL liner Add about 100 μL of n-hexane extraction solvent ...

Embodiment 2

[0098] Gas chromatography-mass spectrometry analysis was carried out on the tobacco leaf sample by using the technical scheme of Example 1. The difference is that the temperature of the micro-extraction in this application is 250° C., and the time of the micro-extraction is 3 min, 4 min and 5 min respectively.

[0099] The result is as Figure 7-9 as shown, Figure 7 The micro-extraction time that the embodiment of the present invention 2 obtains is the chromatogram of 3min, Figure 8 The micro-extraction time that the embodiment of the present invention 2 obtains is the chromatogram of 4min, Figure 9 The micro-extraction time that obtains for the embodiment 2 of the present invention is the chromatogram of 5min. Depend on Figure 7-9 It can be seen that when the micro-extraction time is 4 minutes, the types and quantities of aroma components are the largest.

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Abstract

The invention provides a preparation method of a tobacco composition gas chromatography sample. The preparation method comprises the following steps: performing airflow purging micro-extraction on a to-be-tested tobacco sample to obtain an extraction phase, wherein the temperature of the airflow purging micro-extraction is 240-260 DEG C, the time spent on the airflow purging micro-extraction is 3-5min, an extraction solvent used for the airflow purging micro-extraction is normal hexane; mixing the extraction phase with a hydrochloric acid solution, performing liquid-liquid extraction to obtain an organic phase; and removing water in the organic phase to obtain the tobacco composition gas chromatography sample. Compared with a conventional method, the tobacco composition gas chromatography sample obtained by adopting the method disclosed by the invention does not nearly contain nicotine, and the problems of limited chromatographic peak capacity, insufficiency of resolution, and the like due to coverage of chromatographic peaks of partial compounds are avoided; according to the preparation method, the loss of partial volatile components is prevented, the rapid and accurate extraction of multiple aroma components in tobacco leaves is realized, and the detection sensitivity and the resolution of the aroma components of the tobacco leaves are improved.

Description

technical field [0001] The invention relates to the technical field of tobacco component analysis, in particular to a method for preparing a tobacco component gas chromatographic analysis sample and a tobacco component gas chromatographic analysis method. Background technique [0002] The aroma of tobacco is the comprehensive experience of human physiological senses on the aroma and taste of tobacco. The aroma of tobacco is the main content of evaluating the quality of tobacco, and it is closely related to the chemical composition of tobacco. The aroma of tobacco is the result of the joint action of various aroma components with aroma characteristics. There are many aroma components, and the interaction of various aroma components with very small content forms the overall sensory quality of different tobaccos. In order to reveal the aroma composition of tobacco, Tobacco aroma has been studied tirelessly. [0003] The currently known method for detecting tobacco components i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 崔龙吉金玉善邹依霖李东浩周萍崔月刘金霞
Owner JILIN TOBACCO IND
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