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Test strip for specific cephalosporin antibiotic allergen ige screening and preparation method thereof

A technology of test strips and allergens, which is applied in the field of test strips and its preparation for IgE screening of specific cephalosporin antibiotic allergens, and can solve problems such as cumbersome operation, poor sensitivity and precision, and long test time , to achieve the effect of simple operation, accurate results and low cost

Active Publication Date: 2016-01-13
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are disadvantages of cumbersome operation, poor sensitivity and precision, and long test time

Method used

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Examples

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Embodiment Construction

[0027] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0028] Example for the test strip and preparation method thereof for specific cephalosporin antibiotic allergen IgE screening

[0029] The preparation of test strips for IgE screening of specific ceftizoxime allergens includes the preparation of the antigen solution to be labeled, the preparation of the colloidal gold solution to be labeled, the colloidal gold-labeled ceftizoxime antigen, and the purification of the colloidal gold ceftizoxime antigen , Particle size identification of colloidal gold protein particles and coating of test strips. Specific steps are as follows:

[0030] 1. Preparation of antigen solution to be labeled

[00...

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Abstract

The invention provides a test strip for screening specific cephalosporin antibiotic allergen IgE (Immunoglobulin E). The test strip is coated by a cephalosporin antibiotic antigen labeled by colloidal gold. The colloidal gold labelling technology is utilized in the invention; the specific cephalosporin antibiotic allergen is labelled by using colloidal cold for the first time; then, the labelled allergen is uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane, therefore, the test strip is prepared for detecting allergens; the test strip disclosed by the invention is simple and convenient to operate, accurate in result and low in cost; and a number of free tests can be realized.

Description

technical field [0001] The invention relates to the technical field of colloidal gold labeling, in particular to a test strip for screening specific cephalosporin antibiotic allergen IgE and a preparation method thereof. Background technique [0002] Allergic disease is the most common disease in contemporary society. It is caused by widespread allergens. About 5-10% of the population in my country and 5-25% of the population in the United States and Europe have suffered from allergic infestation. Among them, young children And teenagers are particularly obvious, and harm is more serious. Allergic reaction (allergy) is a pathological immune response that causes physiological dysfunction and tissue damage when the immune body is exposed to the same allergen again. Involves various clinical departments, such as internal medicine including bronchial asthma, anaphylactic shock, serum sickness, connective tissue disease, glomerulonephritis, migraine, etc., surgery including trans...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/531
CPCG01N33/531G01N33/558G01N2800/24
Inventor 蒋琳
Owner BIOSINO BIO TECH & SCI
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