Treatment and prevention of diseases related to oxidative stress
A technology for oxidative stress and disease conditions, applied in metabolic diseases, blood diseases, extracellular fluid diseases, etc.
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[0167] Materials and methods
[0168] basic material
[0169] Insulin, epinephrine, p-, m-, o-tyrosine, and Mg2SO4 were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO 63178, USA) . NaCl, KCl, KH2PO4, NaHCO3, glucose and CaCl2*2H2O were purchased from Merck (Merck KGaA, 64271 Darmstadt, Germany).
[0170] cell culture
[0171] Primary cultures of mouse embryonic fibroblasts (3T3-L1) were purchased from ATCC (Manassas, USA). Supplemented with 10% fetal bovine serum (Gibco (Gibco), CAT number: 16170-078), 100U / ml penicillin and 0.1mg / ml streptomycin (Gibco, CAT number: 15070-063) and 398nM Dulbecco's modified Eagle medium (Dulbecco's modified Eagle medium, DMEM, Invitrogen, CAT number: 41966-029; Sigma-Aldrich CAT number: D6046) of p-, m- or o-Tyr Early passage of 3T3-L1 fibroblasts. DMEM contains 25 mM or 5 mM glucose (depending on the experiment), L-glutamine and pyruvate. Cells were grown in a humidified incubator at 37°C and 5% CO2. The differentiation med...
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