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Petroleum hydrocarbon degradation bacteria DLFJ1-1 as well as degradation gene and application thereof

A petroleum hydrocarbon degrading bacteria and petroleum hydrocarbon technology are applied in the field of environmental petroleum pollution biological treatment, which can solve the problems of destroying marine productivity, reducing the service function and value of marine ecosystems, and lack of oxygen.

Inactive Publication Date: 2014-08-27
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Oil spill pollution accidents will also cause severe hypoxia in large areas of the sea, destroy marine productivity, cause a large number of fish, shrimp, shellfish and seabirds to die, and cause local "ocean desertification"; Cause deserted beaches, destroy mariculture and salt pan production, pollute or destroy coastal tourist areas, and reduce the service function and value of marine ecosystems

Method used

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  • Petroleum hydrocarbon degradation bacteria DLFJ1-1 as well as degradation gene and application thereof
  • Petroleum hydrocarbon degradation bacteria DLFJ1-1 as well as degradation gene and application thereof
  • Petroleum hydrocarbon degradation bacteria DLFJ1-1 as well as degradation gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Isolation and identification of Thioclava sp.DLFJ1-1

[0017] The surface seawater in the oil-spill area of ​​Xingang, Dalian was collected on site, and seawater culture medium was added with 1% petroleum: diesel oil = 1:1 for enrichment culture at 28°C and 180r / min. One week later, the plates were diluted in solid medium (M2) to obtain multiple strains of pure bacteria, which were inoculated into liquid medium to verify their ability to degrade petroleum hydrocarbons, and finally a highly efficient petroleum hydrocarbon-degrading bacterium DLFJ1 was obtained -1, the bacterium was identified as Thioclava sp. by 16S rDNA; the similar sequence was selected, and the phylogenetic distance of the sequence was calculated with MEGA5.0, and the phylogenetic tree was constructed. The phylogenetic tree showed that the strains with the highest similarity were all Thioclava sp. strains. Combined with the determination results of 16S rDNA of the strain, it was assigned t...

Embodiment 2

[0018] Embodiment two: Thioclava sp.DLFJ1-1 degrades the performance of petroleum hydrocarbon

[0019] Inoculate Thioclava sp.DLFJ1-1 into seawater medium containing 1% petroleum: diesel oil = 1:1, culture in a shaker at 180r / min and 28°C in the dark, and take samples every other day to determine the culture medium by gravimetric method The degradation rate of petroleum hydrocarbons, see the experimental results figure 1 .

[0020] Depend on figure 1 It can be seen that Thioclava sp.DLFJ1-1 can degrade 1% petroleum:diesel oil=1:1 within 7 days by 59.195% within 7 days.

Embodiment 3

[0021] Example three: Antibiotic susceptibility test of Thioclava sp.DLFJ1-1

[0022] The antibiotic susceptibility test adopts the disc method, and the antibacterial diameter of each antibiotic disc on the MA plate of the cultured degrading bacteria is used as the judgment standard of sensitivity or resistance (see Table 2).

[0023] Table 2 DLFJ1-1 antibiotic experiments

[0024]

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Abstract

The invention discloses petroleum hydrocarbon degradation bacteria DLFJ1-1 as well as a degradation gene and application thereof, relating to environmental petroleum pollution biotreatment. The petroleum hydrocarbon degradation bacteria (Thioclava sp.) DLFJ1-1 have a collection number CGMCC (China General Microbiological Culture Collection Center) No.9013. Organic compounds are petroleum hydrocarbon compounds; the petroleum hydrocarbon compounds comprise but are not limited to hydrocarbon compounds such as diesel oil and liquid alkane. Numerous genes related to degradation of alkane or aromatic hydrocarbons are discovered by performing genome sequencing on the Thioclava sp. DLFJ1-1 and can be applied to petroleum hydrocarbon polluted environment monitoring and bioremediation. An experiment proves that the petroleum hydrocarbon degradation bacteria DLFJ1-1 can grow and propagate by virtue of the petroleum and the diesel oil as energy resources and carbon sources and are capable of degrading 59.195% of 5g / L of the petroleum and the diesel oil in an inorganic salt culture medium within 7 days, and the ratio of the petroleum to the diesel oil is 1:1.

Description

technical field [0001] The invention relates to biological treatment of environmental petroleum pollution, in particular to a strain of petroleum hydrocarbon degrading bacteria DLFJ1-1 and its degradation gene and application. Background technique [0002] Petroleum and its products are the most important energy and industrial raw materials for human beings, known as "industrial blood" and "blood of human civilized society". However, while oil has brought great impetus to the development of human society, it has also caused non-negligible oil spill pollution and ecological damage to the human environment, especially the marine environment. Oil spill pollution accidents will also cause severe hypoxia in large areas of the sea, destroy marine productivity, cause a large number of fish, shrimp, shellfish and seabirds to die, and cause local "ocean desertification"; Cause deserted beaches, destroy mariculture and salt pan production, pollute or destroy coastal tourist areas, an...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32
CPCY02W10/37
Inventor 邵宗泽王万鹏张荣秋单大鹏
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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