Large-scale seedling culturing technology for rhododendron calophytum Franch

Abstract

The invention discloses a large-scale seedling culturing technology for rhododendron calophytum Franch. The technology includes the following steps that seeds, collected in the last year, of the rhododendron calophytum Franch are placed in a refrigerator and stored to March in the second year at the temperature of 4 DEG C; the seeds are treated for 24 hours through 600 mg / l GA3 and then flushed for 10 minutes through running water; the mature and full seeds are selected and placed in a culture dish where absorbent gauze and filter paper are arranged in a cushioning mode, then the culture dish is placed in a culture box for culturing, wherein the culture box is at the low temperature of 20 DEG C, illumination is performed according to 16 h / 8 h (light / dark), water is sprayed one time per day, and the rate of emergence of the seedlings can reach over 95% after two weeks; all the emerged seedlings are transplanted into a plastic green house and placed in matrixes for planting, wherein peat and perlite are mixed in the substrates, and then water is sprayed every day. A lot of rhododendron calophytum Franch plants are obtained; the resources of wild rhododendron calophytum Franch in Qinling mountains are conserved and protected; the large-scale seedling culturing technology lays the foundation for seedling culturing of the rhododendron calophytum Franch; material stock is provided for hybridization seed culturing of existing monotonous rhododendron varieties in China.

Description

technical field [0001] The invention relates to a seedling raising technology, in particular to a large-scale seedling raising technology of rhododendron beautifense. Background technique [0002] Rhododendron calophytum Franch. is an evergreen shrub of Rhododendron calophytum Franch. Among the few Rhododendron plants in the Qinling Mountains, it has high ornamental value and breeding value because of its huge inflorescence, rich variation and early flowering period. But its introduction and propagation is very difficult, because of its high growth altitude, so the survival rate of seedlings introduced directly is very low, cutting propagation and grafting propagation are also almost infeasible, tissue culture propagation is more difficult to solve the problem of explant disinfection and sterilization, so Seed propagation has become the only way for it to reproduce in large quantities and protect its resources, and it can also be used as a method for seedling breeding of th...

AI Technical Summary

Problems solved by technology

But its introduction and propagation is very difficult, because of its high growth altitude, so the survival rate of seedlings introduced directly is very low, cutting propagation and grafting propagation are also almost infeasible, tissue culture propagation is more difficult to solve the problem of explant disinfection and sterilization, so Seed propagation has become the only way to reproduce in large quantities and protect its resources, and it can also be used as a method for seedling breeding of this species

The existing rhododendrons are sown and propagated directly in the field with sand, but because the seeds of rhododendrons are extremely small, direct watering after sowing tends to compact the soil, resulting in seeds not germinating; in addition, according to experiments, rhododendrons do not germinate under high temperature Or the germination rate is extremely low, even if there is no gibberellin treatment at low temperature, a small amount of seeds will germinate

Therefore, if the field is covered with film, the temperature inside the film will be too high, and the germination rate of seedlings will be zero.

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