Kit for detecting mycobacterium tuberculosis infection and monitoring clinical treatment effect and application of kit
A technology for detection of Mycobacterium tuberculosis and infection, applied in the field of biomedical testing, can solve the problem of the decline in the number of tuberculosis antigen-specific immune cells
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0049] Example 1: Detection of antigen-specific IP-10 by intracellular cytokine staining.
[0050] The experimental steps are as follows:
[0051] 1. The peripheral blood of patients with active tuberculosis was collected in anticoagulant tubes, and separated by Ficoll density gradient centrifugation to obtain PBMCs.
[0052] 2. PBMCs were washed twice with 5-10ml PBS or RPMI1640, resuspended in RPMI1640 containing 10% FBS, counted the number of cells, and adjusted to a final concentration of 2.5×10 6 / ml.
[0053] 3. Add 100 μl of cell suspension to each well of a 96-well cell culture plate, that is, 0.25×10 6 cell. Mycobacterium tuberculosis-specific antigens or polypeptides were added to the experimental wells, the final concentration of the antigen or single polypeptide was 10 μg / ml, and the final concentration of each polypeptide in the polypeptide library was 2 μg / ml. Add 2.5 μg / ml PHA to the positive control wells, and add the same volume of RPMI1640 medium containi...
Embodiment 2
[0064] Example 2: Detection of antigen-specific IP-10 by ELISA.
[0065] The experimental steps are as follows:
[0066] 1. The peripheral blood of patients with active tuberculosis, patients with latent tuberculosis infection, normal people and non-tuberculosis pulmonary disease controls were collected in anticoagulant tubes, and separated by Ficoll density gradient centrifugation to obtain PBMCs.
[0067] 2. PBMCs were washed twice with 5-10ml PBS or RPMI1640, then resuspended in RPMI1640 containing 10% FBS, counted the number of cells, and adjusted to a final concentration of 2.5×10 6 / ml.
[0068] 3. Add 100 μl of cell suspension to each well of a 96-well cell culture plate, that is, 0.25×10 6 PBMC cells. Mycobacterium tuberculosis-specific antigens or polypeptides were added to the experimental wells, the final concentration of the antigen or single polypeptide was 10 μg / ml, and the final concentration of each polypeptide in the polypeptide library was 2 μg / ml. Add 2....
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com