Preparation method of bitter almond polysaccharide and bitter almond oligosaccharide

A technology of bitter almonds and oligosaccharides, which is applied in the field of preparation of bitter almond polysaccharides and oligosaccharides, can solve the problems of no public reports, no reports on the research of bitter almond oligosaccharides, and few studies, and achieve raw materials Rich, good social and economic benefits, simple preparation method

Inactive Publication Date: 2014-09-10
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research on its active ingredients mainly involves amygdalin, protein, enzymes, flavonoids, fatty oils and other ingredients. There are few studies on polysaccharides, and the research on amygdalin oligosaccharides has not been reported, and how to solve the integrated preparation There is no public report on bitter almond polysaccharides and oligosaccharides

Method used

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  • Preparation method of bitter almond polysaccharide and bitter almond oligosaccharide
  • Preparation method of bitter almond polysaccharide and bitter almond oligosaccharide
  • Preparation method of bitter almond polysaccharide and bitter almond oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The present invention in concrete implementation, comprises the following steps:

[0013] (1) Take peeled bitter almonds, add petroleum ether 5 times the weight of bitter almonds, place in a flash extractor to extract for 1 min, filter to obtain bitter almond dregs A, volatilize bitter almond dregs A to remove petroleum ether, Dry in the air, add 78% ethanol with a volume concentration 6 times the weight of bitter almonds, soak for 30 min, reflux at 50°C for 0.5 h, filter to obtain the extract and bitter almond dregs B, and the bitter almond dregs B for later use, extract 40 Centrifuge at 4000r / min for 15 minutes to get the supernatant which is the extract of bitter almond oligosaccharides, pass the extract of bitter almonds oligosaccharides through AB-8 macroporous adsorption resin column, activated carbon column, strongly acidic styrene-based cation-exchange resin column, and strongly basic styrene-based anion-exchange resin column to obtain an eluent, and finally con...

Embodiment 2

[0016] The present invention in concrete implementation, comprises the following steps:

[0017] (1) Take peeled bitter almonds, add petroleum ether 7 times the weight of bitter almonds, place in a flash extractor to extract for 2.7min, filter to obtain bitter almond dregs A, volatilize bitter almond dregs A to remove petroleum ether , dry, add bitter almond weight 8.5 times the volume concentration of 79% ethanol, soak for 30min, reflux extraction at 55°C for 2h, filter to obtain the extract and bitter almond dregs B, the bitter almond dregs B for later use, the extract 45 Ethanol was evaporated under reduced pressure at ℃, centrifuged at 4000r / min for 15min, and the supernatant obtained was the extract of bitter almond oligosaccharides. The extract of bitter almond oligosaccharides was passed through D-101 macroporous adsorption resin column and activated carbon in sequence. column, strongly acidic styrene-based cation-exchange resin column, and strongly basic styrene-based ...

Embodiment 3

[0020] The present invention in concrete implementation, comprises the following steps:

[0021] (1) Take peeled bitter almonds, add petroleum ether 10 times the weight of bitter almonds, place in a flash extractor for extraction for 3 minutes, filter to obtain bitter almond dregs A, volatilize bitter almond dregs A to remove petroleum ether, Dry in the air, add 15 times the weight of bitter almonds in ethanol with a volume concentration of 82%, soak for 30 minutes, reflux at 65°C for 3 hours, filter to obtain the extract and bitter almond dregs B, the bitter almond dregs B for later use, extract at 50°C Ethanol was evaporated under reduced pressure, centrifuged at 4000r / min for 15min, and the supernatant obtained was the extract of bitter almond oligosaccharides, and the extract of bitter almond oligosaccharides was passed through AB-8 macroporous adsorption resin column and activated carbon column in sequence 1. Strongly acidic styrene-based cation-exchange resin column, str...

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Abstract

The invention relates to a preparation method of bitter almond polysaccharide and bitter almond oligosaccharide. Through the preparation method, preparation of the bitter almond polysaccharide and the bitter almond oligosaccharide can be effectively achieved, and application of the prepared bitter almond polysaccharide in preparing medicine and health care products for boosting immunity and application of the prepared bitter almond oligosaccharide in preparing oxidation-resisting medicine, oxidation-resisting health care products, oxidation-resisting food additives and oxidation-resisting cosmetics can be achieved. The preparation method is simple, operation is easy, raw materials are abundant, and product application range is wide; bitter almonds are used as raw materials to prepare the bitter almond polysaccharide and the bitter almond oligosaccharide, the application range of bitter almond medicinal materials is widened, the application value of the bitter almond polysaccharide and the bitter almond oligosaccharide is developed, and the bitter almond polysaccharide and the bitter almond oligosaccharide have good social benefits and economic benefits and are a huge innovation in traditional Chinese medicine.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of bitter almond polysaccharide and oligosaccharide. Background technique [0002] Bitter almond, alias: almond, is the dry mature seed of Prunus armeniaca L.var.ansu Maxim., Prunus sibirica L., Prunus mandshurica (Maxim.) Koehne or Prunus armeniaca L. of Rosaceae plants. It has the effects of reducing qi, relieving cough and asthma, laxative, anti-inflammatory and analgesic, anti-tumor, hypoglycemic, and hypolipidemic. Clinically, it is mainly used for cough and asthma, chest fullness and phlegm, intestinal dryness and constipation. The current research on its active ingredients mainly involves amygdalin, protein, enzymes, flavonoids, fatty oils and other ingredients. There are few studies on polysaccharides, and the research on amygdalin oligosaccharides has not been reported, and how to solve the integrated preparation There is no public report on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H3/06C07H1/08A61P37/04A61P39/06A61Q19/02A23L1/09A23L1/30
Inventor 刘富岗冯素香吴兆宇王蒙蒙李晓玉郝蕊
Owner HENAN UNIV OF CHINESE MEDICINE
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