Method for prolonging fermentation period of glutamic acid strain
A fermentation cycle, glutamic acid technology, applied in the field of microbial fermentation, can solve problems such as no reports, and achieve the effect of prolonging the fermentation cycle and expanding the space
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Embodiment 1
[0021] Example 1 A method for prolonging the fermentation period of glutamic acid strains, taking the fermentation liquid at the peak of acid production by glutamic acid fermentation as the screening object, screening its activity rapidly decreased with the mother slant, and then expanding the culture as the original species, Specific steps are as follows:
[0022] a. When fermenting in the running fermenter in production for 9 hours, take 10ml of fermented liquid with a sterile test tube, the pH of the fermented liquid is stable at 7.0, the carbon-nitrogen ratio is controlled at 100:15, sterilized by moist heat method, and the sample is taken for sterility test ;
[0023] b. Recover the strains in the fermented liquid obtained in a from the fermented acid production stage to the growth stage, that is, the growth temperature of the strains is rapidly dropped from 38.5°C to 33.5°C, and the fermented liquid is kept at a constant temperature to 33.5°C;
[0024] c. Screen the str...
Embodiment 2
[0027] Embodiment 2 A kind of method that prolongs the fermentation cycle of glutamic acid strain, concrete steps are as follows:
[0028] a. When fermenting in the running fermenter in production for 13 hours, use a sterile test tube to take 30ml of fermented liquid. The pH of the fermented liquid is stable at 8.0, and the carbon-nitrogen ratio is controlled at 100:33. Sterilize with damp heat method, and take samples for sterility inspection ;
[0029] b. Recover the strains in the fermented liquid obtained in a from the fermented acid production stage to the growth stage, that is, the growth temperature of the strains is rapidly dropped from 38.5°C to 33.5°C, and the fermented liquid is kept at a constant temperature to 33.5°C;
[0030] c. Screen the strains that have returned to the growth phase in b, and select the strains that have strong activity and high differentiation rate to be expanded and cultivated as fermentation strains for production; use this fermentation liq...
Embodiment 3
[0033] Embodiment 3 A kind of method that prolongs the fermentation cycle of glutamic acid strain, concrete steps are as follows:
[0034] a. When fermenting in the running fermenter for 11 hours, use a sterile test tube to take 20ml of fermented liquid. The ph of the fermented liquid is stable at 7.5, and the carbon-nitrogen ratio is controlled at 100:24. Sterilize with damp heat method, and take samples for sterility test ;
[0035] b. Recover the strains in the fermented liquid obtained in a from the fermented acid production stage to the growth stage, that is, the growth temperature of the strains is rapidly dropped from 38.5°C to 33.5°C, and the fermented liquid is kept at a constant temperature to 33.5°C;
[0036] c. Screen the strains that have returned to the growth phase in b, and select the strains that have strong activity and high differentiation rate to be expanded and cultivated as fermentation strains for production; use this fermentation liquid as the mother sl...
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