A method for the detection of indole alkaloids in toad venom
A detection method and indole technology, applied in the field of analysis and detection of traditional Chinese medicine components, to achieve the effects of quality control, qualitative and quantitative analysis methods synchronization, and good stability
Active Publication Date: 2016-04-13
HEHUANG PHARMA SHANGHAI
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Problems solved by technology
Therefore, it is necessary to adopt optimized pretreatment and detection conditions to establish a method for the determination of indole alkaloid components in Bufoni venom: 5-hydroxytryptamine and bufotryptamine. The shortcoming of insufficient specificity can control the quality of Chansu more comprehensively and effectively
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Embodiment 1
[0048] Example 1 Determination of two kinds of indole alkaloids in the toad venom medicinal material
[0049] 1 Experimental part
[0050] 1.1 Sample pretreatment
[0051] Preparation of the test solution: Accurately weigh 0.1g of toad venom fine powder, accurately add 50% ethanol aqueous solution, weigh, ultrasonically extract, after cooling, weigh again, make up for weight loss with 50% ethanol aqueous solution, shake well. A centrifuge is used for centrifugation, the centrifugation time is 20min, and the centrifugation speed is 4000rpm. Then accurately draw 3ml of the supernatant, place it in an evaporating dish, heat it, and evaporate it to nearly dryness. Add 20% ethanol aqueous solution to dissolve and set the volume to 10ml, pass through a 0.45μm filter membrane, and take the subsequent filtrate as the test solution.
[0052] Preparation of reference substance solution: Accurately weigh an appropriate amount of 5-hydroxytryptamine and bufotryptamine reference substan...
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Abstract
The invention provides a method for detecting a fingerprint spectrum of indole alkaloid components in venenum bufonis medicinal materials. The method mainly comprises the following steps: preparing and measuring a test solution and a reference solution, and comparing liquid chromatograms of the test solution and the reference solution; and determining two indole alkaloid components in the test solution according to relative retention time, and determining the content of the two indole alkaloid components in the test solution according to chromatographic peak area and by an external standard method. The method can perform effective qualitative and quantitative analysis on the two main indole alkaloid components in venenum bufonis simultaneously, is simple, convenient, feasible and accurate, is high in precision, repeatability and stability, and can be used for controlling the quality of venenum bufonis effectively and providing reference for clinical medication of venenum bufonis.
Description
technical field [0001] The invention relates to the technical field of analysis and detection of traditional Chinese medicine components, in particular to a detection method for indole alkaloid components in toad venom. Background technique [0002] The 2010 edition of "Chinese Pharmacopoeia (Part One)" records that toad venom is a medicinal material obtained by processing and drying the white serum secreted by the retroauricular glands and skin glands of Bufobufogargarizans Cantor or Bufomelanostictus Schneider. Its properties are oblate lumps or flakes. The lumps are hard and not easy to break. The cross-section is brown, horny and slightly shiny; the flakes are brittle and fragile, and the cross-section is reddish brown and translucent. . The taste of toad's supper is sweet at first, and then has a long-lasting spicy feeling. The powder smells like a sneeze. It has the effects of detoxification, pain relief, resuscitation and refreshment. It is mainly used to treat carb...
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IPC IPC(8): G01N30/02
Inventor 顾佳敏姜鹏陈忠樑詹常森向阳朱佳娴许雯雯
Owner HEHUANG PHARMA SHANGHAI