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Detection method for mitochondria damages of nasopharynx mucosae cells

A detection method, mucosal cell technology, applied in the detection field, can solve the problems of complex operation, expensive equipment, difficult implementation, etc., and achieve the effect of high sensitivity and low cost

Inactive Publication Date: 2014-09-17
QINGDAO BELLO BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of complex operation, expensive equipment and difficult implementation in the method for detecting mitochondrial damage of nasopharyngeal mucosa cells in the prior art, the present invention provides a method for detecting free ferrous protoporphyrin in nasopharyngeal exudate cells, and further understanding The method for detecting whether the mitochondria of nasopharyngeal mucosal cells are damaged, the method provided by the present invention is simple and easy to implement, with low cost, and can quickly screen whether the mitochondria of nasopharyngeal mucosal cells are damaged

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Test samples as follows:

[0016] 1) Take 0.5-1 ml of nasopharyngeal exudate;

[0017] 2) Put the nasopharyngeal exudate into the sampling bottle pre-installed with buffer solution, and mix well; the sample bottle is pre-installed with acetic acid buffer solution with a pH value of 4.5, the amount of buffer solution is 2ml, and mix well to obtain the sample solution ;

[0018] 3) Add 1ml of reagent A and 1ml of reagent B to the sample bottle in sequence; mix well, observe the color change within 2 minutes, and compare the test results with the color guide;

[0019] The reagent A is bis(4.dimethylaminophenyl)methane with a concentration of 0.002mol / L, which is prepared into a solution after being dissolved in methanol;

[0020] The reagent B is 1.5% hydrogen peroxide solution.

[0021] 【judgement standard】

[0022] The liquid in the sample bottle has no color or is slightly blue (1) Negative

[0023] blue (+) positive

[0024] dark blue (++) stron...

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Abstract

The invention provides a detection method for mitochondria damages of nasopharynx mucosae cells. The detection method comprises the following detection steps: taking nasopharynx seepage; putting the nasopharynx seepage into a sampling bottle which is prefilled with a buffering solution and uniformly mixing; adding 1ml of a reagent A and 1ml of a reagent B into the sample bottle in sequence; uniformly mixing and observing the color change within two minutes; and comparing a detection result with a colorimetric plate. The detection method is simple and rapid and can be used for detecting the result that the concentration of the lowest free ferrous protoporphyrin can be up to 5ng / ml; the sensitivity is very high.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method for mitochondrial damage of nasopharyngeal mucosal cells. Background technique [0002] Mitochondria are the most important organelles in human cells and the "power factories" of cells. The detection of mitochondria in damaged nasopharyngeal mucosa cells includes the detection of mitochondrial permeability transition pore (mitochon2dria permeability transition pore, MPTP), the detection of membrane phospholipids, the detection of membrane potential, the detection of 2mtDNA damage, the detection of intracellular calcium ion concentration, etc. This type of detection operation is very complicated and requires sophisticated instruments and equipment, which is difficult to complete in general laboratories, which greatly limits the detection of mitochondrial damage in nasopharyngeal mucosal cells. Before the above difficult detection, use a simple and low-cost method to initia...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 温鑫崔子伦
Owner QINGDAO BELLO BIOTECHNOLOGY CO LTD
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