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Method and system suitable for high-speed continuous super-resolution positioning and imaging

An imaging method and super-resolution technology, applied in the field of super-resolution imaging, can solve the problems that cannot meet the needs of rapid image processing and visualization functions, and achieve the effect of satisfactory image analysis speed and rapid analysis

Active Publication Date: 2014-09-24
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

[0003] However, the existing super-resolution positioning imaging methods are far from meeting the fast image processing and visualization functions required by biological applications. Therefore, it is important to explore a real-time image processing method and system suitable for high-speed continuous super-resolution positioning imaging. of great concern

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  • Method and system suitable for high-speed continuous super-resolution positioning and imaging
  • Method and system suitable for high-speed continuous super-resolution positioning and imaging

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specific Embodiment approach

[0052] A specific implementation of this step includes:

[0053]The switch state of the activation light and the excitation light is controlled by the shutter SH; the power of the activation light and the excitation light is adjusted by the attenuation sheet ND; the activation light and the excitation light are combined into one beam by the first dichroic mirror DM1; by the first convex lens L1 Expand the combined laser beam with the second convex lens L2; ​​focus the expanded laser beam through the third convex lens L3; shoot it on the surface of the sample through the objective lens, so that the fluorescent probe in the sample emits fluorescence; the fluorescent probe emits The fluorescence is collected by the objective lens. It should be noted here that the fluorescence information collected by the objective lens includes: fluorescence information emitted from sparsely distributed fluorescent probes and fluorescent information emitted from densely distributed fluorescent pr...

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Abstract

The invention relates to the technical field of super-resolution imaging, and discloses a method and a system suitable for high-speed continuous super-resolution positioning and imaging. The method comprises the steps of performing denoising and de-overlapping on an original acquired image to obtain an image to be processed; positioning a sub-pixel position coordinate of a brighter imaging molecule from the image to be processed by a radiation symmetric method, and obtaining an airy disk model on the sub-pixel position coordinate of the brighter imaging molecule; then subtracting the airy disk model on the sub-pixel position coordinate of the brighter imaging molecule by the image to be processed to obtain a sub-pixel position coordinate of a darker imaging molecule, and finally displaying the sub-pixel position coordinates of the imaging molecules to realize real-time quick analysis, processing and displaying on a super-resolution positioning image, so that the requirement on biological application on an image analysis speed is met.

Description

technical field [0001] The invention relates to the technical field of super-resolution imaging, and is mainly applicable to a high-speed continuous super-resolution positioning imaging method and system. Background technique [0002] Super-resolution positioning imaging system has become an important and potential tool in biological applications because it breaks through the diffraction limit to achieve nanoscale resolution and can be used to observe living cells. Typical super-resolution localization imaging systems are Photo-activated Localization Microscopy (PALM) and Stochastic Optical Reconstruction Microscopy (STORM). [0003] However, the existing super-resolution positioning imaging methods are far from meeting the fast image processing and visualization functions required by biological applications. Therefore, it is important to explore a real-time image processing method and system suitable for high-speed continuous super-resolution positioning imaging. received ...

Claims

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Application Information

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IPC IPC(8): G01N21/64G06T5/00G06T7/00
Inventor 黄振立马洪强李梦婷曾绍群
Owner HUAZHONG UNIV OF SCI & TECH
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