The invention discloses a super-resolution confocal ophthalmoscope based on an optical pupil filter and a dark field technique. The super-resolution confocal ophthalmoscope is capable of accurately acquiring super-resolution dark field images of retinas of living human eyes in real time, and comprises a beacon light source, an imaging light source, an optical pupil filter, a two-dimensional scanning galvanometer, a Hartmann sensor, a deformable mirror, an optical filter and a photoelectric detection system. The beacon light source is used for correcting human eye aberrations; the imaging light source is used for acquiring human eye images. The operating method comprises the following steps: characterizing resolution according to Rayleigh criterion by using full width at half maximum, adding a two-zone type phase optical pupil filter at an illumination end, wherein the pinhole is equal to Airy disk size; allowing the pinhole to be equal to 1.5 times that of Airy disk size when the filter(s) is / are arranged at the imaging end or two ends, so that the diffraction limit condition can be realized when the transverse full width at half maximum is less than the pinhole of a general microscope equal to the Airy disk size, and the super-resolution image can be acquired at a super-resolution ratio. On the basis, the pinhole equal to the Airy disk size is translated by an Airy disk distance, the pinhole equal to 1.5 times that of the Airy disk size is subjected to central blocking or linear blocking, so that dark field imaging can be realized.