Super-resolution confocal ophthalmoscope based on optical pupil filter and dark field technique

Abstract

The invention discloses a super-resolution confocal ophthalmoscope based on an optical pupil filter and a dark field technique. The super-resolution confocal ophthalmoscope is capable of accurately acquiring super-resolution dark field images of retinas of living human eyes in real time, and comprises a beacon light source, an imaging light source, an optical pupil filter, a two-dimensional scanning galvanometer, a Hartmann sensor, a deformable mirror, an optical filter and a photoelectric detection system. The beacon light source is used for correcting human eye aberrations; the imaging light source is used for acquiring human eye images. The operating method comprises the following steps: characterizing resolution according to Rayleigh criterion by using full width at half maximum, adding a two-zone type phase optical pupil filter at an illumination end, wherein the pinhole is equal to Airy disk size; allowing the pinhole to be equal to 1.5 times that of Airy disk size when the filter(s) is / are arranged at the imaging end or two ends, so that the diffraction limit condition can be realized when the transverse full width at half maximum is less than the pinhole of a general microscope equal to the Airy disk size, and the super-resolution image can be acquired at a super-resolution ratio. On the basis, the pinhole equal to the Airy disk size is translated by an Airy disk distance, the pinhole equal to 1.5 times that of the Airy disk size is subjected to central blocking or linear blocking, so that dark field imaging can be realized.

Description

technical field [0001] The invention relates to a medical imaging diagnosis system for imaging the retina, in particular to a super-resolution confocal ophthalmoscope based on pupil filter and dark field technology. Background technique [0002] The retina is a membrane about 300 microns thick located in the fundus of the human eye. It includes a multi-layer structure such as nerve fiber layer, nerve cell layer, blood vessel layer, visual cell layer and melanocyte epithelial cell layer. The human retina is an indispensable and important information in ophthalmology diagnosis and treatment. Real-time tracking of the details of the fundus retina will help the early diagnosis and prevention of physical diseases. [0003] In 1987, R.H.Webb applied confocal scanning technology to imaging of the retina of the living human eye. Since the living human eye is equivalent to an optical system, there are various aberrations, which greatly limit the resolution and contrast of retinal im...

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is: ordinary self-adaptive confocal ophthalmoscope, due to the restriction of the diffraction limit, the lateral width at half maximum of PSF cannot be further reduced; , the efficiency is low and more detailed information of the retina cannot be obtained

Images