Comprehensive evaluation method for passive cutaneous anaphylaxis test

A technology for skin allergies and reactions, applied in biological testing, material inspection products, testing pharmaceutical preparations, etc., can solve the problems of insufficient accuracy and high false negative rate, and achieve the effect of comprehensive evaluation indicators and avoiding false negatives

Inactive Publication Date: 2014-09-24
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a more accurate method for judging the drug in preclinical pharmacological experiments in view of the shortcomings of insufficient accuracy and high false negative rate of the currently commonly used judgment method for judging the occurrence of passive skin allergy. Evaluation method of whether it can cause passive skin allergic reaction in animals

Method used

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  • Comprehensive evaluation method for passive cutaneous anaphylaxis test
  • Comprehensive evaluation method for passive cutaneous anaphylaxis test
  • Comprehensive evaluation method for passive cutaneous anaphylaxis test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of evaluation method for passive allergic reaction experiment, the steps are as follows:

[0020] 1. Preparation of antiserum for passive skin allergic reaction test: Male BN rats were randomly divided into normal saline group and test drug group. Each group was sensitized on the 1st, 3rd, and 5th day, respectively, for a total of three times, and the animals in each group were subcutaneously given the clinically commonly used dose of the corresponding drug each time. Each group was challenged on the 12th day after the last sensitization, and the blood was collected before the challenge, and the supernatant was collected after centrifugation at 3000r / min for 15min, and stored at -20°C for future use.

[0021] 2. Passive skin allergy test: SD rats were randomly divided into normal saline group and test drug group, with 8 rats in each group. Rats were shaved at a place about 1.5cm away from the back midline on both sides of the back, with an area of ​​about 4×4cm....

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Abstract

The invention discloses a comprehensive evaluation method for a passive cutaneous anaphylaxis test. The method particularly comprises the steps that (1), an antigen is injected according to the passive cutaneous anaphylaxis, and a tester observes the expression of common symptoms of a rat with naked eyes after the antigen is injected; (2), the toe swelling degree of the rat after being injected with the antigen is calculated; (3), blood is taken, and blood and the biochemical criterions of the rat are observed; (4), the rat is killed, and the tester observes whether locus coeruleus appears in the skin of the back of the rat or not, and the skin, the toes and the ears of the rat and the OD value of the blood are detected.

Description

technical field [0001] The invention relates to a method for evaluating the occurrence of an allergic reaction, specifically a method for comprehensively and comprehensively examining the occurrence of an allergic reaction through changes in multiple indicators. Background technique [0002] Traditional Chinese medicine injection is an original modern new dosage form in my country. It has the advantages of high bioavailability, fast onset, strong and wide-ranging effects, outstanding curative effect, and convenient use. It is widely used in the treatment of various diseases. With the widespread use of traditional Chinese medicine injections, reports of adverse reactions have also increased. Although the reported adverse reactions of traditional Chinese medicine injections involve multiple systems, the most common is allergic reactions. Data show that the frequency of adverse drug reactions in traditional Chinese medicine injections in clinical use is significantly higher tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15G01N33/48
Inventor 蒋宝平余黎许立田磊
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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