Detection kit for auxiliary diagnosis on diabetes and application method of detection kit
A detection kit and auxiliary diagnosis technology, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem of non-disclosure, etc., and achieve the effect of high accuracy, high accuracy and reducing false positive results.
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[0042] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0043] A detection kit for auxiliary diagnosis of diabetes, said detection kit comprising primers for detecting single nucleotide polymorphisms of NFKBIE gene, CD36 gene and ADIPOQ gene, said single nucleotide polymorphisms are respectively rs2233424, rs1194182 and rs266729, the primers include:
[0044] NFKBIE gene rs2233424 specific upstream primer one:
[0045] 5'-gcgggcagggcggcCCCAAAGATGCCAGCTTGTACg-3';
[0046] NFKBIE gene rs2233424 specific upstream primer two:
[0047] 5'-gattaccgTCCCGGATCATTCATTTGTTTTAAAAGa-3';
[0048] NFKBIE gene rs2233424 downstream primer:
[0049] 5'-CTTTTAAAACAAATGAATGATCCGGGA-3';
[0050] CD36 gene rs1194182 specific upstream primer one:
[0051] 5'-gcgggcagggcggcGTCCGAAGGCTGGAAGGCTc-3';
[0052] CD36 gene rs1194182 specific upstream primer two:
[0053] 5'-gattaccgCTGACATCTGAAAGCCATTGACATCCTg-3';
[00...
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