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Detection kit for auxiliary diagnosis on diabetes and application method of detection kit

A detection kit and auxiliary diagnosis technology, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem of non-disclosure, etc., and achieve the effect of high accuracy, high accuracy and reducing false positive results.

Inactive Publication Date: 2014-10-01
NINGBO UNIV
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Problems solved by technology

However, at present, there is no published method for detecting the rs2233424 single nucleotide polymorphism of the NFKBIE gene, the rs1194182 single nucleotide polymorphism of the CD36 gene, the rs266729 single nucleotide polymorphism of the ADIPOQ gene and the type 2 insulin pathway. Relevant research reports on kits related to diabetes

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  • Detection kit for auxiliary diagnosis on diabetes and application method of detection kit
  • Detection kit for auxiliary diagnosis on diabetes and application method of detection kit
  • Detection kit for auxiliary diagnosis on diabetes and application method of detection kit

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Embodiment Construction

[0042] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0043] A detection kit for auxiliary diagnosis of diabetes, said detection kit comprising primers for detecting single nucleotide polymorphisms of NFKBIE gene, CD36 gene and ADIPOQ gene, said single nucleotide polymorphisms are respectively rs2233424, rs1194182 and rs266729, the primers include:

[0044] NFKBIE gene rs2233424 specific upstream primer one:

[0045] 5'-gcgggcagggcggcCCCAAAGATGCCAGCTTGTACg-3';

[0046] NFKBIE gene rs2233424 specific upstream primer two:

[0047] 5'-gattaccgTCCCGGATCATTCATTTGTTTTAAAAGa-3';

[0048] NFKBIE gene rs2233424 downstream primer:

[0049] 5'-CTTTTAAAACAAATGAATGATCCGGGA-3';

[0050] CD36 gene rs1194182 specific upstream primer one:

[0051] 5'-gcgggcagggcggcGTCCGAAGGCTGGAAGGCTc-3';

[0052] CD36 gene rs1194182 specific upstream primer two:

[0053] 5'-gattaccgCTGACATCTGAAAGCCATTGACATCCTg-3';

[00...

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Abstract

The invention discloses a detection kit for auxiliary diagnosis on diabetes. The detection kit comprises primers for detecting single nucleotide polymorphisms of a NFKBIE gene, a CD36 gene and an ADIPOQ gene, wherein the single nucleotide polymorphisms are respectively rs2233424, rs1194182 and rs266729. According to the invention, polymerase chain reaction (PCR) amplification is performed on sample DNA by using the primers of the detection kit, a melting curve of a PCR amplification product is analyzed by using a fluorogenic quantitative PCR instrument, the melting curve is further compared with a standard melting curve, the Tm value of the PCR amplification product is in comparison, and then a corresponding genetic typing result is obtained. The detection kit can be applied to auxiliary detection and prediction on diabetes of a subject and is simple in detection method, high in detection efficiency and high in pertinence, early prevention of diabetes is promoted, and reference is provided for medicine treatment.

Description

technical field [0001] The invention relates to the technical field of auxiliary diagnosis of diabetes, in particular to a detection kit for auxiliary diagnosis of diabetes and a use method thereof. Background technique [0002] Diabetes mellitus is a common endocrine and metabolic disease. It is caused by absolute or relative insufficiency of insulin in the blood, resulting in hyperglycemia and glycosuria, which in turn causes fat and protein metabolism disorders. Clinically, polyuria, polydipsia, polydipsia, Hyperphagia, emaciation and other manifestations, severe cases are prone to acute complications such as ketoacidosis or chronic complications such as blood vessels and nerves. Diabetes is one of the most serious diseases threatening human health today, and it is the main killer of human health. With the improvement of living conditions, the prevalence of diabetes in my country is increasing year by year, and is gradually approaching that of developed countries. Accor...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6883C12Q2600/156C12Q2531/113C12Q2527/107C12Q2563/107
Inventor 段世伟汤琳琳麦一峰叶孟
Owner NINGBO UNIV
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