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Giant porphyrin-phospholipid vesicles

A technology of vesicles and porphyrins, applied in the direction of microcapsule preparations, using microcapsules, enzymes, etc., can solve the problems that the process is not easily controllable, hinders high-viscosity solvents, and the method is difficult to control

Inactive Publication Date: 2014-10-01
UNIV HEALTH NETWORK
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

6 While highly feasible for some applications, these methods are difficult to control
The opening and closing of inflated large lipid vesicles is well characterized, but the process is not easily controllable and often uses highly viscous solvents that hinder many applications

Method used

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  • Giant porphyrin-phospholipid vesicles
  • Giant porphyrin-phospholipid vesicles
  • Giant porphyrin-phospholipid vesicles

Examples

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Embodiment

[0054] method

[0055] Unless otherwise stated, all chemical materials were purchased from Sigma and all electronic materials were purchased from Mouser. GPVs are formed using a modified electroforming method. 12 Pyropheophytin-lipid (prepared as previously 11 , but with a modified protocol to generate the isomerically pure conjugate; manuscript submitted) was dispersed in chloroform to form a 0.2 mg / ml-0.5 mg / ml stock solution. Two 0.5 mm diameter platinum wires (No. 267228, Sigma) were placed 2 mm apart in parallel through a Teflon O-ring (No. 9559K208, McMaster-Carr) attached to a cover slide using vacuum lubricant. ). 6-10 evenly spaced 1 μl droplets of stock solution were placed on two platinum wires. Unless otherwise indicated, 70 mole % pyropheophorbide-lipid was used. The remaining chloroform was evaporated by placing the O-ring device under vacuum for 20 minutes. The lipid on the wire was then hydrated with 0.6 mL of an aqueous solution of Tris with 2 mM pH 8. ...

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Abstract

There is provided herein vesicles comprising a bilayer comprising porphyrin-phospholipid conjugate, wherein the porphyrin-phospholipid conjugate comprises one porphyrin, porphyrin derivative or porphyrin analog covalently attached to a lipid side chain, preferably at the sn-1 or the sn-2 position, of one phospholipids, wherein the vesicle is 1-100 microns in diameter.

Description

[0001] related application [0002] This application claims priority to US Provisional Patent Application No. 61 / 568,352, filed December 8, 2011. technical field [0003] The present invention relates to the field of porphyrin-phospholipid vesicles, and more particularly, to macroporphyrin-phospholipid vesicles capable of spatially and temporally controlled opening and closing. Background technique [0004] Phospholipid closed compartments play a central role in cellular and subcellular homeostasis, where the bilayer acts as a general barrier between external and internal biomolecules and chemicals. The postulated prebiological bilayer is already understanding and modeling how cells control biomolecules 1-3 The passage and resulting background are reproduced. A wide range of protein-based transport systems have evolved in organisms to allow molecules to move across the bilayer without disrupting the integrity of the overall membrane. However, these delivery systems are of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68B01J13/02C12N11/04C12P1/00C12Q1/00C12N15/88
CPCC12N11/04G01N1/28C12N15/88B01J13/02C12Q1/68C12Q1/6806C12N13/00C12Q1/00C12P1/00Y10T436/143333A61K41/0028
Inventor 郑岗乔纳森·洛弗尔伊丽莎白·许恩
Owner UNIV HEALTH NETWORK
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