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Method for deducting postmortem interval of human body

A technology of time and human body, applied in the field of medical examination, can solve the problem of no gallbladder reflecting the interval time after death, and achieve the effect of easy extraction and storage, overcoming subjectivity, and ensuring accuracy

Active Publication Date: 2014-10-08
JIANGHAN UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem that the gallbladder does not reflect the postmortem interval in the prior art, the embodiment of the present invention provides a method for inferring the postmortem interval of the human body

Method used

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  • Method for deducting postmortem interval of human body
  • Method for deducting postmortem interval of human body
  • Method for deducting postmortem interval of human body

Examples

Experimental program
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Embodiment

[0036] 30 cases of isolated human gallbladder tissues with exact death time in Hubei in 2013 were selected. The age of the deceased was mostly 30±10 years old. Among them, there were 28 cases of males (93.33% of the total) and 2 cases of females (6.67% of the total). ). The cadavers before sampling were stored in an environment of 16-25°C. The temperature of the experimental environment for sampling was 14.5-25°C. The first collection of samples was completed 5 hours after the death of the human body.

[0037] The complete gallbladder was taken out under sterile surgical conditions, placed in normal saline containing 400mg / L gentamicin, stored in a 18°C ​​incubator for 0.5h, and then cut 0.5 1 cm × 0.5 cm × 1 cm sample, and cut it once every 1 hour, immerse the cut sample in clean water and shake to wash, clean the plasma components and blood cells, take out the sample and dry it with filter paper, hold it with tweezers and dry it The sample of the sample was smeared and appl...

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Abstract

The invention discloses a method for deducting the postmortem interval of a human body, and belongs to the medical science examination field. The method comprises the following steps: extracting human gallbladder mucous membrane epithelial cells at different death times as samples, and smearing the samples; dyeing the smeared samples; carrying out image analysis on the dyed samples, wherein analysis parameters comprise index of density, gray scale parameters and geometric parameters of the nuclei of the gallbladder mucous membrane epithelial cells, and the gray scale parameters comprise average optical density, average gray scale and integral optical density; and carrying out statistics on the parameters to obtain linear regression equations of the parameters, and determining the postmortem interval according to one or more of the linear regression equations. The index of density, gray scale parameters and geometric parameters of the nuclei of the gallbladder mucous membrane epithelial cells are analyzed within 5-36h after human body death through method for deducting the postmortem interval of the human body to obtain a relation between each of the index of density, gray scale parameters and geometric parameters and PMI in order to deduct the postmortem interval of the human body.

Description

technical field [0001] The invention relates to the field of medical examination, in particular to a method for estimating the interval time after death of a human body. Background technique [0002] The postmortem interval (PMI) estimation of the human body has always been one of the key points, difficulties and hot spots in the research of forensic pathology, especially the early PMI inference, which is used in judicial practice such as screening criminal suspects in criminal investigation and arbitrating death medical disputes. more realistic. Objective and accurate early PMI inference can provide important objective clues for criminal investigations by public security organs, which is helpful for public security organs to identify whether criminal suspects have time to commit crimes, determine or reduce the scope of investigation, and improve the efficiency and accuracy of investigations. In addition, the inference of the interval after death is sometimes crucial for th...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCG01N1/30G01N15/0227G01N21/84G01N15/01
Inventor 李长雷杨友华易卉玲舒细记
Owner JIANGHAN UNIVERSITY
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