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Extracting method, detection method and application of total alkaloids in sophora tonkinensis

A technology of total alkaloids and extraction method, which is applied in the field of extraction of total alkaloids in pagoda japonica, can solve the problem of the reduction of mountain bean root resources, and achieve the effects of easy control, simple extraction process, and easy industrial production

Active Publication Date: 2014-10-15
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of the gradual decrease of the root resources in the current market, and to provide an extraction method, a detection method and an application thereof for total alkaloids in Sophora japonica, so as to explore whether the medicinal material of Sophora japonica can be used as A substitute for bean root, providing a scientific basis for resource utilization and clinical application

Method used

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  • Extracting method, detection method and application of total alkaloids in sophora tonkinensis
  • Extracting method, detection method and application of total alkaloids in sophora tonkinensis
  • Extracting method, detection method and application of total alkaloids in sophora tonkinensis

Examples

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Effect test

Embodiment 1

[0023] 1-1 Grind the multi-leaf pagoda japonica medicinal material into a 20-mesh coarse powder, reflux extraction with 70% ethanol at 90°C for 5 times, each time for 1 hour, filter while it is hot, and recover the solvent to obtain a compound with a relative density of 1.20 at 60°C Fluid extract: add water to suspend the fluid extract, add dropwise concentrated sulfuric acid until the pH value is 3; extract with chloroform until the color of chloroform becomes lighter, add potassium carbonate to the acidic water layer until the pH value is 11, extract with chloroform until the chloroform liquid is steamed There is no alkaloid reaction after drying, and the chloroform is recovered to obtain the chloroform extract, which is the total alkaloid.

[0024] 1-2 Crush the multi-leaf pagoda japonica medicinal material into 80-mesh coarse powder, reflux extraction with 75-90% ethanol at 85°C for 4 times, each time for 2 hours, filter while it is hot, and recover the solvent, and the rel...

Embodiment 2

[0027] 2-1 Grind 20 kg of leafy pagoda japonica medicinal material into coarse powder, pass through a 20-mesh sieve, put it into a percolator, compact it layer by layer, and then put 160 kg of prepared 1% hydrochloric acid aqueous solution into the percolator, Sealed, extract for 7 days, do not stir during the percolation process, collect the percolate; pass the percolate through the pre-treated 732 cation exchange resin column, and then wash the resin column with water until the water is colorless; pour the resin into the open In a container, dry it in the air or dry it at 55-65°C, knead it with ammonia water, extract it with chloroform reflux 5 times, and evaporate the chloroform extract to dryness to check that there is no alkaloid reaction, and recover the chloroform extract to obtain the chloroform extract, namely Get total alkali.

[0028] 2-2 Grind 15 kg of leafy pagoda japonica medicinal material into coarse powder, pass through a 20-mesh sieve, put it into a percolato...

Embodiment 3

[0034] Get 10 mg of the total alkaloids of Pagoda japonica polyphylla obtained by the extraction method of Example 1 or 2, put it in a 10ml volumetric flask, add chloroform to the mark, shake it up, and use it as the test solution; Add 10ml of chloroform, 0.2ml of concentrated ammonia test solution, shake for 15 minutes, filter, evaporate the filtrate to dryness, add 0.5ml of chloroform to dissolve the residue, and use it as a reference medicinal solution; Reference substance of reference substance, oxidized sophocarpine reference substance plus chloroform to make a mixed solution containing 1mg per 1ml, as the reference substance solution, test according to thin-layer chromatography, draw the test solution, and control medicinal material solution 1~ 2 μl, 4~6 μl of the reference substance solution, respectively spot on the same silica gel G thin-layer plate, use chloroform-methanol-concentrated ammonia test solution (4:1:0.1) as the developer, develop, take out, and dry in the...

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Abstract

The invention discloses an extracting method, a detection method and application of total alkaloids in sophora tonkinensis. The extracting method comprises two ways: (1) conducting heating, backflow and extraction on sophora tonkinensis medicinal material by 70-95% ethyl alcohol to obtain fluid extract; conducting extraction with chloroform after watering suspension and acid adjusting treatment to obtain the total alkaloids; (2) conducting percolating extraction on the sophora tonkinensis medicinal material; conducting elution extraction by 732 cation exchange resin; conducting chloroform elution treatment on the resin to obtain the total alkaloids. The obtained total alkaloids are detected through a thin layer chromatography; trichloromethane-methyl alcohol-dense ammonia detection solution (4:1:0.1) is adopted as a developing solvent; sophocarpidine, oxymatrine and oxidized sophocarpine are taken as determined ingredients in the total alkaloids; the result is accurate and reliable. According to the invention, the extracting method is simple in process; the obtained total alkaloids are provided with pharmacologic action of gastric ulcer resistance, anticoagulation, cerebral ischemia resistance, antithrombus property, blood fat reduction, and the like, and are provided with relatively broad market development prospect.

Description

technical field [0001] The invention belongs to the technical field of medicinal liquid extraction, and in particular relates to an extraction method, a detection method and an application of total alkaloids in pagoda japonica. Background technique [0002] Leafy Sophora japonica Sophora tonkinensis Gagnep.var. polyphylla S. Z.Huang et Z.C.Zhou is a plant of the genus Sophora in the family Fabaceae, and it is combined with the commonly used Chinese medicinal material Shandougen [scientific name: Vietnam Sophora japonica Sophora tonkinensis Gagnep] is the root of the same family, genus and species, and it is a variant of Sophora japonica. It grows in Shishan District, and is distributed in Guangxi, Guangdong, Yunnan, Guizhou and Jiangxi provinces. In Guangxi, it is mainly distributed in the Hongshui River Basin, such as Du'an, Mashan, Xincheng, Wuming and other places. It is cold in nature, bitter in taste, and has the effects of clearing heat and detoxifying, reducing sw...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P1/04A61P7/02A61P9/10A61P3/06G01N30/90
Inventor 卢文杰陈家源陆国寿谭晓黄建猷黄周锋何开家李燕婧钟正贤
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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